| Yazarlar |
Fatma Beyazit
Faculty of Medicine, Çanakkale |
|
Mehmet Yakup Arica
Gazi University, Teknikokullar |
|
Ilkay Acikgoz-Erkaya
Faculty of Engineering and Architecture, Kırsehir |
|
Cengiz Ozalp
School of Medicine, Ankara |
|
Gulay Bayramoglu
Gazi University, Teknikokullar |
| Özet |
| A fast and accurate identification of Listeria monocytogenes. A new quartz crystal microbalance (QCM) aptasensor was designed for the specific and rapid detection of L. monocytogenes. Before detection of the target bacterium from samples in the QCM aptasensor, a magnetic pre-enrichment system was used to eliminate any contaminant in the samples. The prepared magnetic system was characterized using ATR-FTIR, SEM, VSM, BET, and analytical methods. The saturation magnetization values of the FeO, FeO@PDA, and FeO@PDA@DAPEG particles were 57.2, 40.8, and 36.4 emu/g, respectively. The same aptamer was also immobilized on the QCM crystal integrated into QCM flow cell and utilized to quantitatively detect L. monocytogenes cells from the samples. It was found that a specific aptamer-magnetic pre-concentration system efficiently captured L. monocytogenes cells in a short time (approximately 10 min). The FeO@PDA@DA-PEG-Apt particles provided selective isolation of L. monocytogenes from the bacteria-spiked media up to 91.8%. The immobilized aptamer content of the magnetic particles was 5834 µg/g using 500 ng Apt/mL. The QCM aptasensor showed a very high range of analytical performance to the target bacterium from 1.0 × 10 and 1.0 × 10 CFU/mL. The limit of detection (LOD) and limit of quantitation (LOQ) were 148 and 448 CFU/mL, respectively, from the feeding of the QCM aptasensor flow cell with the eluent of the magnetic pre-concentration system. The reproducibility of the aptasensor was more than 95%. The aptasensor was very specific to L. monocytogenes compared to the other Listeria species (i.e., L. ivanovii, L. innocua, and L. seeligeri) or other tested bacteria such as Staphylococcus aureus, Escherichia coli, and Bacillus subtilis. The QCM aptasensor was regenerated with NaOH solution, and the system was reused many times. |
| Anahtar Kelimeler |
| Listeria monocytogenes detection, Aptasensor, Magnetic particles, Pre-concentration, Quartz crystal microbalance |
| Makale Türü | Özgün Makale |
| Makale Alt Türü | Uluslararası alan indekslerindeki dergilerde yayımlanan tam makale |
| Dergi Adı | Mikrochimica acta |
| Dergi ISSN | 1436-5073 |
| Makale Dili | İngilizce |
| Basım Tarihi | 04-2024 |
| Cilt No | 191 |
| Sayfalar | 235 / 0 |
| Doi Numarası | 10.1007/s00604-024-06307-2 |
| Atıf Sayıları |
