| Yazarlar (5) |
Meliha Koldemir Gündüz
Türkiye |
|
Güllü Kaymak
Türkiye |
Dr. Öğr. Üyesi Ertan KANBUR
Kırşehir Ahi Evran Üniversitesi, Türkiye |
|
Derya Berikten
Kütahya Sağlık Bilimleri Üniversitesi, Türkiye |
|
Harun Şener
Kütahya Sağlık Bilimleri Üniversitesi, Türkiye |
| Özet |
| Considering the rapidly increasing prevalence of obesity worldwide, the number of weight control drugs is very few. Incretin-based therapies are currently being developed to achieve weight control, and Glucagon-Like Peptide-1 Receptor Agonists (GLP-1RA) are used in incretin-based therapies. This study aimed to investigate the cytotoxicity of exenatide, a GLP-1A, on 3T3-L1 adipocytes and the effect of exenatide on the expression of adipogenesis-related genes, insulin and glucose levels, and apoptosis. Cytotoxic activity of exenatide on 3T3-L1 adipocytes was determined by MTT method. Gene expression levels were determined by qPCR. Apoptosis studies were performed on the Muse Cell Analyzer. C1q/TNF-related protein-3 (CTRP3) expression levels were found to be higher in exenatide treated adipocyte cells than in control cells (p < 0.001). Adipocyte cells treated with exenatide were found to have lower PPAR-gamma gene expression levels when compared to control adipocyte cells (p < 0.001). Intracellular insulin (p < 0.001) and glucose levels were higher in 3T3-L1 adipocytes treated with exenatide compared to control adipocyte cells. Total apoptosis increased approximately 1.5 times as a result of exenatide administration. The increase in CTRP3 gene expression, which is thought to be a new biomarker for obesity, and the decrease in PPAR-gamma gene expression indicate that exenatide is a promising new pharmaco-therapeutic agent in the treatment of obesity by regulating the expression of genes related to adipogenesis and lipogenesis and inducing apoptosis. |
| Anahtar Kelimeler |
| Obesity | Adipogenesis | 3T3-L1 cell line | Exenatide | CTRP3 gene |
| Makale Türü | Özgün Makale |
| Makale Alt Türü | SSCI, AHCI, SCI, SCI-Exp dergilerinde yayımlanan tam makale |
| Dergi Adı | TOXICOLOGY IN VITRO |
| Dergi ISSN | 0887-2333 Wos Dergi Scopus Dergi |
| Dergi Tarandığı Indeksler | SCI-Expanded |
| Dergi Grubu | Q2 |
| Makale Dili | Türkçe |
| Basım Tarihi | 12-2022 |
| Cilt No | 85 |
| Doi Numarası | 10.1016/j.tiv.2022.105479 |
| Makale Linki | http://dx.doi.org/10.1016/j.tiv.2022.105479 |
| Atıf Sayıları | |
| WoS | 1 |
| Google Scholar | 1 |
