High-frequency protocorm-like bodies and shoot regeneration through a combination of thin cell layer and RITA<SUP>®</SUP> temporary immersion bioreactor in Cattleya forbesii Lindl.

Ekmekcigil, Munire; BAYRAKTAR, MELTEM; Akkus, Ozge; Gurel, Aynur

doi:10.1007/s11240-018-1526-2

High-frequency protocorm-like bodies and shoot regeneration through a combination of thin cell layer and RITA^® temporary immersion bioreactor in Cattleya forbesii Lindl.

Yazarlar (4)

Munire Ekmekcigil
Ege Üniversitesi, Türkiye

Doç. Dr. Meltem BAYRAKTAR Kırşehir Ahi Evran Üniversitesi, Türkiye

Ozge Akkus Muğla Sıtkı Koçman Üniversitesi, Türkiye

Prof. Dr. Aynur Gurel Ege University Faculty Of Engineering, Türkiye

Makale Türü	Özgün Makale (SSCI, AHCI, SCI, SCI-Exp dergilerinde yayınlanan tam makale)
Dergi Adı	Plant Cell Tissue and Organ Culture (Q2)
Dergi ISSN	0167-6857 Dergi Bilgileri (2019)
Makale Dili	İngilizce	Basım Tarihi	03-2019
Cilt / Sayı / Sayfa	136 / 3 / 451–464	DOI	10.1007/s11240-018-1526-2
UAK Araştırma Alanları	Ziraat, Orman ve Su Ürünleri

Özet

An efficient in vitro mass propagation through protocorm-like bodies (PLBs) was established in Cattleya forbesii Lindl., a commercially important orchid. Whole PLBs (W-PLB) and transverse thin cell layers of PLB (tTCL-PLB) explants were cultured in RITA^® bioreactors based on temporary immersion system. Explants were transferred in semi-solid or RITA^® bioreactor for protocorm production or shoot regeneration. The effect of different immersion frequencies, medium volumes and inoculum densities were studied and optimized. RITA^® bioreactor cultures were found to be superior compared with semi-solid cultures regarding PLB production and shoot regeneration. tTCL-PLB explant types cultured in the RITA^® bioreactor with immersion for 1 min/4 h, 250 mL of medium and 20 explants showed the highest number of PLBs per RITA^® (2237 PLBs) and per explant (111.9 PLBs). The highest number of PLBs per explant was 21 times higher than those from semi-solid culture. The highest number of shoots per RITA^® (3998 shoots) and per explant (199.9 shoots) were observed on tTCL-PLB cultured in RITA^® bioreactor (1 min/4 h; 150 mL of medium and 20 explants). The highest number of shoots per explant was 95 times higher than those grown on semi-solid culture. Mass propagation of PLBs and shoots of C. forbesii Lindl. using combined thin cell layer and RITA^® temporary immersion has been adapted in commercial practice.

Anahtar Kelimeler

BM Sürdürülebilir Kalkınma Amaçları

Atıf Sayıları

High-frequency protocorm-like bodies and shoot regeneration through a combination of thin cell layer and RITA^® temporary immersion bioreactor in Cattleya forbesii Lindl.

Dergi Adı	PLANT CELL TISSUE AND ORGAN CULTURE
Kısa Adı	PLANT CELL TISS ORG
Yayıncı	SPRINGER
Açık Erişim	Hayır
ISSN	0167-6857
E-ISSN	1573-5044
Wos Quartile	Q2
Scopus Quartile	Q1
Tarandığı Indeksler	SCIE , Scopus
WoS Kategoriler	BIOTECHNOLOGY & APPLIED MICROBIOLOGY \| PLANT SCIENCES
Scopus Kategoriler	HORTICULTURE

High-frequency protocorm-like bodies and shoot regeneration through a combination of thin cell layer and RITA® temporary immersion bioreactor in Cattleya forbesii Lindl.

Paylaş

High-frequency protocorm-like bodies and shoot regeneration through a combination of thin cell layer and RITA^® temporary immersion bioreactor in Cattleya forbesii Lindl.