High-frequency protocorm-like bodies and shoot regeneration through a combination of thin cell layer and RITA<SUP>®</SUP> temporary immersion bioreactor in Cattleya forbesii Lindl.

Ekmekcigil, Munire; BAYRAKTAR, MELTEM; Akkus, Ozge; Gurel, Aynur

doi:10.1007/s11240-018-1526-2

High-frequency protocorm-like bodies and shoot regeneration through a combination of thin cell layer and RITA^® temporary immersion bioreactor in Cattleya forbesii Lindl.

Yazarlar
Munire Ekmekcigil
Doç. Dr. Meltem BAYRAKTAR Kurum Bilgileri Mühendislik-Mimarlık Fakültesi Genetik ve Biyomühendislik Genetik ve Biyomühendislik Özgeçmiş Sayfası İletişim Bilgileri: (386)280-3821 Kırşehir Ahi Evran Üniversitesi
Ozge Akkus
Aynur Gurel

Özet

An efficient in vitro mass propagation through protocorm-like bodies (PLBs) was established in Cattleya forbesii Lindl., a commercially important orchid. Whole PLBs (W-PLB) and transverse thin cell layers of PLB (tTCL-PLB) explants were cultured in RITA((R)) bioreactors based on temporary immersion system. Explants were transferred in semi-solid or RITA((R)) bioreactor for protocorm production or shoot regeneration. The effect of different immersion frequencies, medium volumes and inoculum densities were studied and optimized. RITA((R)) bioreactor cultures were found to be superior compared with semi-solid cultures regarding PLB production and shoot regeneration. tTCL-PLB explant types cultured in the RITA((R)) bioreactor with immersion for 1min/4h, 250mL of medium and 20 explants showed the highest number of PLBs per RITA((R)) (2237 PLBs) and per explant (111.9 PLBs). The highest number of PLBs per explant was 21 times higher than those from semi-solid culture. The highest number of shoots per RITA((R)) (3998 shoots) and per explant (199.9 shoots) were observed on tTCL-PLB cultured in RITA((R)) bioreactor (1min/4h; 150mL of medium and 20 explants). The highest number of shoots per explant was 95 times higher than those grown on semi-solid culture. Mass propagation of PLBs and shoots of C. forbesii Lindl. using combined thin cell layer and RITA((R)) temporary immersion has been adapted in commercial practice.

Anahtar Kelimeler

Makale Türü	Özgün Makale
Makale Alt Türü	SSCI, AHCI, SCI, SCI-Exp dergilerinde yayımlanan tam makale
Dergi Adı	PLANT CELL TISSUE AND ORGAN CULTURE
Dergi ISSN	0167-6857
Dergi Grubu	Q2
Makale Dili	İngilizce
Basım Tarihi	03-2019
Cilt No	136
Sayı	3
Sayfalar	451 / 464
Doi Numarası	10.1007/s11240-018-1526-2

BM Sürdürülebilir Kalkınma Amaçları

Atıf Sayıları
WoS	19

High-frequency protocorm-like bodies and shoot regeneration through a combination of thin cell layer and RITA^® temporary immersion bioreactor in Cattleya forbesii Lindl.

Akademisyenler > Meltem BAYRAKTAR > Yayın Detayı

High-frequency protocorm-like bodies and shoot regeneration through a combination of thin cell layer and RITA^® temporary immersion bioreactor in Cattleya forbesii Lindl.

Paylaş

Akademisyenler > Meltem BAYRAKTAR > Yayın Detayı

High-frequency protocorm-like bodies and shoot regeneration through a combination of thin cell layer and RITA® temporary immersion bioreactor in Cattleya forbesii Lindl.

Paylaş

High-frequency protocorm-like bodies and shoot regeneration through a combination of thin cell layer and RITA^® temporary immersion bioreactor in Cattleya forbesii Lindl.