| Yazarlar |
Esra Ilhan-Ayisigi
Kırşehir Ahi Evran Üniversitesi, Turkey |
|
Pelin Saglam-Metiner
Ege University Faculty of Engineering, Turkey |
|
Ebru Sanci
Ege Üniversitesi, Turkey |
|
Buket Bakan
Atatürk Üniversitesi, Turkey |
|
Yeliz Yildirim
Ege Üniversitesi, Turkey |
|
Aylin Buhur
Ege University Medical School, Turkey |
|
Altug Yavasoglu
Ege University Medical School, Turkey |
|
N. Ulku Karabay Yavasoglu
Ege Üniversitesi, Turkey |
|
Ozlem Yesil-Celiktas
Ege University Faculty of Engineering, Turkey |
| Özet |
| Carboplatin (cis-diamine (1,1-cyclobutandicarboxylaso)‑platinum (II)) is a second-generation antineoplastic drug, which is widely used for chemotherapy of lung, colon, breast, cervix, testicular and digestive system cancers. Although preferred over cisplatin due to the lower incidence of nephrotoxicity and ototoxicity, efficient carboplatin delivery remains as a major challenge. In this study, carboplatin loaded alginate- poly(amidoamine) (PAMAM) hybrid nanoparticles (CAPs) with mean sizes of 192.13 ± 4.15 nm were synthesized using a microfluidic platform, then EGF was conjugated to the surface of CAPs (EGF-CAPs) for the receptor-targeted delivery. Hence, increased FITC+ cell counts were observed in A549 spheroids after EGF-CAP treatment compared to CAP in the 3D cellular uptake study. As such, the cytotoxicity of EGF-CAP was approximately 2-fold higher with an IC50 value of 35.89 ± 10.37 μg/mL compared to the CAPs in A549 spheroids. Based on in vivo experimental animal model, anti-tumor activities of the group treated with CAP decreased by 61 %, whereas the group treated with EGF-CAP completely recovered. Additionally, EGF-CAP application was shown to induce apoptotic cell death. Our study provided a new strategy for designing a hybrid nanoparticle for EGFR targeted carboplatin delivery with improved efficacy both in vitro and in vivo applications. |
| Anahtar Kelimeler |
| Alginate | Carboplatin | In vivo lung cancer model | Microfluidic synthesis | PAMAM dendrimer | Targeted drug delivery |
| Makale Türü | Özgün Makale |
| Makale Alt Türü | SCOPUS dergilerinde yayımlanan tam makale |
| Dergi Adı | International Journal of Biological Macromolecules |
| Dergi ISSN | 0141-8130 |
| Makale Dili | İngilizce |
| Basım Tarihi | 03-2024 |
| Cilt No | 261 |
| Sayı | 1 |
| Doi Numarası | 10.1016/j.ijbiomac.2024.129758 |
| Atıf Sayıları |
