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Heavy metal-induced oxidative stress and DNA damage as shown by RAPD-PCR in leaves of Elodea canadensis   
Yazarlar (3)
Dilek Demirezen Yilmaz
Erciyes Üniversitesi, Turkey
Öğr. Gör. Nuri ERCAN Öğr. Gör. Nuri ERCAN
Kırşehir Ahi Evran Üniversitesi, Türkiye
Prof. Dr. Fahriye ERCAN Prof. Dr. Fahriye ERCAN
Kırşehir Ahi Evran Üniversitesi, Türkiye
Devamını Göster
Özet
The objective of the study was to evaluate the antioxidant response and DNA damage of heavy metals (Cd, Cu and Cr) in Elodea canadensis. Superoxide dismutase (SOD), catalase (CAT), Glutathione reductase (GR) and lipid peroxidation levels of leaves of Elodea canadensis which was exposed to different concentrations of heavy metals (Cd: 2, 5, 10, 15, 25 ppb; Cu: 200, 500, 1000, 2500 ppb and Cr: 1, 3, 10, 15, 25 ppb) in a hydroponic culture were determined spectrophotometrically. The highest induction in SOD and CAT activities were determined at highest concentration of heavy metals. The Random Amplified Polymorphic DNA Polymerase Chain Reaction (RAPD-PCR) technique was used to investigate the variation of DNA banding patterns of samples that exposed to different concentrations of heavy metals. Changing in band intensity and the gain and loss of bands were demonstrated the genotoxic effect of heavy metals. Bioaccumulation, oxidative responses and DNA damages were shown that Elodea canadensis represents a useful bioindicator.
Anahtar Kelimeler
DNA Damage | Elodea Canadensis | Oxidative Stress | RAPD-PCR
Makale Türü Özgün Makale
Makale Alt Türü SCOPUS dergilerinde yayımlanan tam makale
Dergi Adı Applied Chemical Engineering
Dergi ISSN 2578-2010
Makale Dili İngilizce
Basım Tarihi 01-2020
Cilt No 3
Sayı 1
Sayfalar 14 / 22
Doi Numarası 10.24294/ace.v3i1.724