The investigation of the presence of some bacterial and viral agents in Pearl mullet (Chalcalburnus tarichii, pallas 1811) by real-time PCR and the histopathological examination
Yazarlar (9)
Özgül Gülaydın
Van Yüzüncü Yıl Üniversitesi, Türkiye
Dr. Öğr. Üyesi Cihat ÖZTÜRK
Van Yüzüncü Yıl Üniversitesi, Türkiye
Şükrü Önalan
Van Yüzüncü Yıl Üniversitesi, Türkiye
Zeynep Karapınar
Balıkesir Üniversitesi, Türkiye
Muhammed Arabacı
Van Yüzüncü Yıl Üniversitesi, Türkiye
İsmail Hakkı Ekin
Van Yüzüncü Yıl Üniversitesi, Türkiye
Ziya İlhan
Balıkesir Üniversitesi, Türkiye
Kemal Gürtürk
Van Yüzüncü Yıl Üniversitesi, Türkiye
Fatma İlhan
Balıkesir Üniversitesi, Türkiye
| Makale Türü | Özgün Makale (SSCI, AHCI, SCI, SCI-Exp dergilerinde yayınlanan tam makale) | ||
| Dergi Adı | FRESENIUS ENVIRONMENTAL BULLETIN (Q4) | ||
| Dergi ISSN | 1018-4619 Wos Dergi Scopus Dergi | ||
| Dergi Tarandığı Indeksler | SCI-Expanded | ||
| Makale Dili | İngilizce | Basım Tarihi | 01-2018 |
| Cilt / Sayı / Sayfa | 27 / 12 / 8286–8296 | DOI | – |
| Makale Linki | https://www.prt-parlar.de/download_feb_2018/ | ||
| Özet |
| The aim of this study was to identify some bacterial (Vibrio cholerae, Listonella anguillarum, Yersinia ruckeri, Aeromonas hydrophila, Aeromonas sobria and Aeromonas caviae) and viral (infectious pancreatic necrosis virus, viral hemorrhagical septicemia virus and infectious hematopoietic virus) pathogens by comparing the bacteriological culture and molecular methods with histopathological examination of various tissues seen in Pearl mullet from freshwater rivers inflowing to the Van lake. Accordingly, bacterial and viral agents were detected in a total of 180 pearl mullet samples by bacteriological culture and Real-Time PCR methods. The samples were collected from six different freshwater rivers that flows to Van lake by random sampling. In the study, Vibrio cholerae, Listonella anguillarum, Yersinia ruckeri, Infectious Pancreatic Necrosis Virus, Viral Hemorrhagic Septicemia Virus and Infectious Hematopoietic Necrosis Virus could not be isolated. However, Aeromonas spp. was identified by bacteriological culture and Real Time PCR methods at 22.22% and 53.33% respectively. The most dominant species was observed to be Aeromonas hydrophila among all identified Aeromonas spp. In histopathological examinations of Aeromonas spp. positive samples, disorders were detected in gill, liver, spleen and kidney tissues. Telangiectasia, edema, hyperplasia and adhesions were observed on the seconder laminates of gills. In liver, the order of the Remak cords were disarranged, and degenerative changes formed in hepatocytes. Melano-macrophages loaded with hemosiderin were intensively detected in all Aeromonas positive samples. As a … |
| Anahtar Kelimeler |
| Bacteria | Virus | Chalcalburnus tarichii | Real-Time PCR | Histopathology | Freshwater rivers flowing into Van lake |
