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The Usability of Extracts of Walnut Green Outer Shell for In vitro Surface Sterilization of Rosehip (Rosa canina L.) Plant   
Yazarlar
Öğr. Gör. Ebru ŞİRİN Öğr. Gör. Ebru ŞİRİN
Türkiye
Doç. Dr. Bahadır ALTUN Doç. Dr. Bahadır ALTUN
Kırşehir Ahi Evran Üniversitesi, Türkiye
Prof. Dr. Ahmet KAZANKAYA Prof. Dr. Ahmet KAZANKAYA
Türkiye
Özet
In this study, it was aimed to determine the efficiency ratios of extracts obtained from walnut outer green shell at different concentrations in order to provide surface sterilization in in vitro propagation of rosehip plant. Axillary buds taken in June were used as explants. After surface sterilization of the explants with walnut outer shell extract, they were cultured in MS0 nutrient medium. The lowest contamination rate on the 5th and 7th days was observed in the control and 20K groups. In addition, the highest number of shoots per explant was obtained in the 20K group. Uncontaminated explants were subcultured to MS0 medium containing 1.0 mg /L IBA + 1.0 mg /L BAP and 1.0 mg/L BAP + 1.0 mg/L NAA at the end of the 7th day. Rooting was not observed in the explants due to browning. In the in vitro propagation of plants, extract (20K) obtained from oven-dried walnut shell can be used instead of chemical sterilant.
Anahtar Kelimeler
Makale Türü Özgün Makale
Makale Alt Türü Ulusal alan endekslerinde (TR Dizin, ULAKBİM) yayımlanan tam makale
Dergi Adı Black Sea Journal of Engineering and Science
Dergi ISSN ISSN: 2618 – 65
Dergi Tarandığı Indeksler TR DİZİN
Makale Dili İngilizce
Basım Tarihi 11-2023
Cilt No 6
Sayı 6
Sayfalar 631 / 635
Doi Numarası 10.47115/bsagriculture.1353925
Makale Linki http://dx.doi.org/10.47115/bsagriculture.1353925