Yazarlar |
Prof. Dr. Atilla TAŞKIN
Kırşehir Ahi Evran Üniversitesi, Türkiye |
Doç. Dr. Fatma ERGÜN
Türkiye |
Ufuk Karadavut
Türkiye |
Demirel Ergün
Türkiye |
Özet |
This study aimed to investigate the usability of different diluents containing 6% Dimethylformamide (DMF) for cryo-preservation of the semen of geese (Anser cygnoides). The diluents of Glucose (G), Tris-Glucose (T), Lactated Ringer’s-Glucose (LG), and Lactated Ringer’s (L), all of which contained 6% DMF, were used as cryoprotectants. The researchers collected semen samples from four geese, twice a week over a four-week period, by means of abdominal massage; they then calculated how much sperm each goose ejaculated. Next, the semen samples were pooled and their spermatological parameters were determined. Their volume (4x (mL)), concentration (×108/mL), pH, motility (%), and vitality (%) rates were 0.31±0.01, 3.49±0.32, 7.13±1.06, 67.75±1.28, and 70.00±2.03, respectively. Then, these pooled semen samples were equally divided into four groups. Once they were frozen and thawed, the researchers discovered that the diluent L had the highest motility rate: 40.12% ± 1.35. The motility rates of the other diluents were as follows: LG (28.25%± 1.48), G (21.50% ± 1.41), and T (5.12% ± 0.83). Likewise, the vitality rates (%) of the diluents were as follows: L (41.93% ±1.87), LG (31.50%±1.88), G (29.43% ±1.45), and T (10.56%±1.34), respectively. Freezing and thawing appeared to lower each diluent’s vitality and motility rates. However, for the Lactated Ringer’s (L), this decrease was predictable. Therefore, Lactated Ringer’s diluent containing 6% DMF can be used in cryo-preservation of goose semen. |
Anahtar Kelimeler |
Cryopreservation | Goose | Semen | Spermatozoa |
Makale Türü | Özgün Makale |
Makale Alt Türü | SSCI, AHCI, SCI, SCI-Exp dergilerinde yayımlanan tam makale |
Dergi Adı | Brazilian Journal of Poultry Science |
Dergi ISSN | 1516-635X |
Dergi Tarandığı Indeksler | SCI-Expanded |
Dergi Grubu | Q3 |
Makale Dili | Türkçe |
Basım Tarihi | 07-2022 |
Cilt No | 24 |
Sayı | 3 |
Sayfalar | 1 / 8 |
Doi Numarası | 10.1590/1806-9061-2020-1429 |
Makale Linki | http://dx.doi.org/10.1590/1806-9061-2020-1429 |