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Effects of different concentrations of honeysuckle (Lonicera iberica M. Bieb.) and barberry (Berberis vulgaris L.) extracts in cryopreserving of duck semen on sperm quality      
Yazarlar
 Atilla TAŞKIN Atilla TAŞKIN
Kırşehir Ahi Evran Üniversitesi, Türkiye
Demirel Ergün
Türkiye
 Fatma ERGÜN Fatma ERGÜN
Kırşehir Ahi Evran Üniversitesi, Türkiye
Özet
The current study examines the effects of honeysuckle (Lonicera iberica M. Bieb.) (Li) and barberry (Berberis vulgaris L.) (Bv) extracts in cryopreserved duck semen on sperm quality due to their proposed antioxidant effects. Of both extracts, 0.1% and 0.2% were added to the duck semen extender and the sperm quality was evaluated after thawing. For this purpose, semen was collected from six 1-year-old drakes by abdominal massage (for 4 weeks, twice a week, 48 ejaculates). Semen volume, sperm density, motility, and viability were determined. An extender containing 6% dimethylformamide (DMF) cryoprotectant (sodium lactate 3 g; sodium chloride 6 g; potassium chloride 0.4 g; calcium chloride dihydrate 0.3 g; dextrose monohydrate (D-glucose) 50 g) and Li and Bv extracts were used. After pooling, collected duck semen samples were randomly divided into 5 equal parts in amount. Then, using an extender at a 1:3 ratio, control (without Li-or Bv-additive), 0.1% Li, 0.2% Li, 0.1% Bv, and 0.2% Bv groups were formed. Semen samples divided into groups were subjected to freezing procedure and preserved in liquid nitrogen. Then, the sperm quality parameters of the groups were assessed after the semen samples were thawed at 37°C. Our findings revealed that the use of plant extracts in different concentrations in the cryopreservation of duck semen can positively affect sperm quality after thawing. Consequently, the extender with 0.1% Bv extract proved to be potentially useful for the long-term preservation of duck semen.
Anahtar Kelimeler
antioxidant | avian sperm | duck | sperm freezing | sperm thawing
Makale Türü Özgün Makale
Makale Alt Türü SSCI, AHCI, SCI, SCI-Exp dergilerinde yayımlanan tam makale
Dergi Adı European Poultry Science
Dergi ISSN 1612-9199
Dergi Tarandığı Indeksler SCI-Expanded
Dergi Grubu Q3
Makale Dili Türkçe
Basım Tarihi 03-2023
Cilt No 87
Sayı 1
Sayfalar 1 / 12
Doi Numarası 10.1399/eps.2023.371
Makale Linki http://dx.doi.org/10.1399/eps.2023.371