img
Correlation between intrahepatic hepatitis B virus cccDNA levels and other activity markers in patients with HBeAg negative chronic hepatitis B infection        
Yazarlar
Rahmet Guner
Prof. Dr. Mustafa Kasım KARAHOCAGİL Prof. Dr. Mustafa Kasım KARAHOCAGİL
Kırşehir Ahi Evran Üniversitesi
Mehmet Buyukberber
Ozlem Kandemir
Onur Ural
Gaye Usluer
Dilara Inan
Iftihar Koksal
Nurcan Baykam
Kenan Hizel
Tansu Yamazhan
Saban Esen
Mehmet A. Tasyaran
Özet
Objective The aim of this study was to demonstrate the relation between intrahepatic (IH) hepatitis B virus (HBV) covalently closed circular DNA (cccDNA) levels and the other HBV replicative intermediates and hepatocyte expression of HBV antigens.
Patients and methods Patients with hepatitis B surface antigen (HBsAg) positivity, hepatitis B early antigen negativity, serum HBV DNA levels 10(4) copies/ml or more, and constantly or intermittently increased alanine aminotransferase levels were included.
Results Fifty-nine patients were included. There was a good correlation between the levels of IH HBV cccDNA and serum HBV DNA (P<0.001). Serum HBsAg levels were weakly correlated with IH HBV cccDNA levels and moderately correlated with serum HBV DNA (r=0.322, P=0.017; r=0.489, P=0.001, respectively). There were no significant correlation between serum HBsAg level and histologic activity index groups (P=0.691), but stage 0, 1, and greater than 2 fibrosis groups were positively correlated with serum HBsAg levels (P=0.019). IH cccDNA and serum HBV DNA were significantly different in hepatitis B core antigen staining groups (P=0.008 and <0.001, respectively) but there was no significant correlation between HBsAg staining groups and HBV replication markers. There was a weak correlation between serum HBsAg levels and IH HBsAg and hepatitis B core antigen levels (r=0.333, P=0.012; r=0.366, P=0.006, respectively). In multivariate analysis, alanine aminotransferase, age, fibrosis stage, and serum HBsAg quantitation were the most important factors predicting IH HBV cccDNA level.
Conclusion Histopathologic damage, serum HBV DNA levels, and IH HBV replication markers have a more complex and dynamic process. However, both serum and IH HBV replication markers provide important knowledge about the activity of the disease. Eur J Gastroenterol Hepatol 23:1185-1191 (C) 2011 Wolters Kluwer Health | Lippincott Williams & Wilkins.
Anahtar Kelimeler
hepatitis B core antigen | hepatitis B early antigen-negative chronic hepatitis | hepatitis B surface antigen | hepatitis B virus | hepatitis B virus covalently closed circular DNA | immunohistochemistry | quantitative hepatitis B surface antigen
Makale Türü Özgün Makale
Makale Alt Türü SSCI, AHCI, SCI, SCI-Exp dergilerinde yayımlanan tam makale
Dergi Adı EUROPEAN JOURNAL OF GASTROENTEROLOGY & HEPATOLOGY
Dergi ISSN 0954-691X
Dergi Tarandığı Indeksler SCI-Expanded
Makale Dili İngilizce
Basım Tarihi 11-2011
Cilt No 23
Sayı 12
Sayfalar 1185 / 1191
Doi Numarası 10.1097/MEG.0b013e32834ba13a
Makale Linki http://content.wkhealth.com/linkback/openurl?sid=WKPTLP:landingpage&an=00042737-201112000-00014