| Yazarlar (12) |
Ezgi Özyılmaz Saraç
Çukurova Üniversitesi, Türkiye |
|
Hacer Sinem Büyüknacar
Çukurova Üniversitesi, Türkiye |
|
Emine Kılıç Bağır
Çukurova Üniversitesi, Türkiye |
|
Leman Sencar Gündoğdu
Çukurova Üniversitesi, Türkiye |
|
Özlem Görüroğlu Öztürk
Çukurova Üniversitesi, Türkiye |
|
İsmail Cem Eray
Çukurova Üniversitesi, Türkiye |
Prof. Dr. Yusuf Kenan DAĞLIOĞLU
Çukurova Üniversitesi, Türkiye |
|
Oya Baydar
Çukurova Üniversitesi, Türkiye |
|
Gülşah Seydaoğlu
Çukurova Üniversitesi, Türkiye |
|
Ufuk Özgü Mete
Çukurova Üniversitesi, Türkiye |
|
Derya Gümürdülü
Çukurova Üniversitesi, Türkiye |
|
Ali Kocabaş
Çukurova Üniversitesi, Türkiye |
| Özet |
| Recent reports have indicated an improved prognosis in sepsis with β-blocker agents; however, the underlying action mechanism is still under debate. The aim of this study was to investigate the potential effect of propranolol on endothelial dysfunction in septic rats. The cecal ligation and puncture model (CLP) was used to generate sepsis. Adult male Wistar-Albino rats were divided into 4 groups: group 1 was a sham group, group 2 received sterile saline, group 3 received 10 mg/kg of propranolol 3 days before the intervention, and group 4 received 10 mg/kg of propranolol 30 min after CLP. Six rats from each group were sacrificed 24 h postoperatively. The remaining rats were followed for survival. We have also evaluated the effects on systemic inflammation, coagulation and the lung tissue with immunohistochemical and electron microscopic evaluation. Serum tumor necrosis factor alpha (TNF-α) and plasminogen activator inhibitor-1 (PAI-1) levels, as well as tissue TNF-α scores were elevated in septic rats. Electron microscopic examination of the lung tissue showed endothelial dysfunction in the sepsis group. Pretreatment significantly improved survival. Moreover, pre-treatment altered serum vascular endothelial growth factor receptor-1 (VEGFR-1) levels and post-treatment reduced serum PAI-1 and VEGFR-1 levels. In both the preand post-treatment groups, electron microscopic examination revealed improvement of the destroyed lung endothelium and showed only mild alterations in the cytoplasmic organelles, especially in the mitochondria of the endothelial cells. These results suggest that the improved outcome with β-blockers in sepsis may be due to the ameliorated endothelial dysfunction. Further studies focusing on the potential effect of β-blockers on the endothelium may lead to a better understanding of sepsis. |
| Anahtar Kelimeler |
| sepsis | endothelial dysfunction | experimental | beta-blockers |
| Makale Türü | Özgün Makale |
| Makale Alt Türü | SSCI, AHCI, SCI, SCI-Exp dergilerinde yayımlanan tam makale |
| Dergi Adı | Advances in Clinical and Experimental Medicine |
| Dergi ISSN | 1899-5276 |
| Dergi Tarandığı Indeksler | SCI-Expanded |
| Dergi Grubu | Q4 |
| Makale Dili | İngilizce |
| Basım Tarihi | 01-2019 |
| Cilt No | 28 |
| Sayı | 3 |
| Sayfalar | 291 / 297 |
| Doi Numarası | 10.17219/acem/78248 |
| Makale Linki | http://www.advances.umed.wroc.pl/en/article/2019/28/3/291/ |
| Atıf Sayıları | |
| WoS | 4 |
| Google Scholar | 5 |
