Yazarlar (4) |
![]() Akdeniz Üniversitesi, Türkiye |
![]() Türkiye |
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![]() Türkiye |
Özet |
A small scale ethylmethanesulfonate (EMS) mutation was used to obtain resistant mutant plants to bacterial canker disease caused by Clavibacter michiganensis subsp. michiganensis isolate 2 (Cmm2). Susceptible EBR3 tomato line (200) seeds were mutagenised with the chemical EMS. Of the constructed M2 population, 450 seedlings were inoculated with Cmm2. Among the tested M2 plants, 15 M2 plants, not affected by the bacterial pathogen, produced M3 family seeds. 10 seeds from each M3 family were re-inoculated with the Cmm2, M3-9 and M3-15 family plant did not segregate for resistance confirming their resistant phenotypes. The M3-9 and M3-15 family mutant plants were analyzed for secondary metabolites especially phenolics; chlorogenic acid, caffeic acid, p-coumaric acid, rutin hydrate, ferulic acid, quercetin, naringenin and caempherol level in high performance liquid chromatography (HPLC) |
Anahtar Kelimeler |
Makale Türü | Özgün Makale |
Makale Alt Türü | Diğer hakemli uluslarası dergilerde yayınlanan tam makale |
Dergi Adı | African Journal of Biotechnology |
Dergi ISSN | 1684-5315 |
Dergi Tarandığı Indeksler | CAB Abstracts |
Makale Dili | İngilizce |
Basım Tarihi | 04-2012 |
Cilt No | 11 |
Sayı | 32 |
Sayfalar | 8070 / 8075 |
Doi Numarası | 10.5897/AJB12.367 |
Atıf Sayıları | |
Google Scholar | 7 |