The proteome of monoclonal antibody purified haustoria from Puccinia triticina Race 1
 
Yazarlar (4)
Christof Rampitsch
Agriculture And Agri-Food Canada, Kanada
Prof. Dr. Aslıhan GÜNEL Kırşehir Ahi Evran Üniversitesi, Türkiye
Eva Beimcik
Agriculture And Agri-Food Canada, Kanada
Wayne Mauthe
Agriculture And Agri-Food Canada, Kanada
Makale Türü Özgün Makale (SSCI, AHCI, SCI, SCI-Exp dergilerinde yayınlanan tam makale)
Dergi Adı Proteomics
Dergi ISSN 1615-9853 Wos Dergi Scopus Dergi
Dergi Tarandığı Indeksler SCI
Makale Dili İngilizce Basım Tarihi 03-2015
Kabul Tarihi 12-04-2026 Yayınlanma Tarihi
Cilt / Sayı / Sayfa 15 / 7 / 1307–1315 DOI 10.1002/pmic.201400241
Özet
Puccinia triticina causes leaf rust, a disease that causes annual yield losses in wheat. It is an obligate parasite that invades the host leaf and forms intracellular structures called haustoria, which obtain nutrients and suppress host immunity using secreted proteins called effectors. Since effector proteins act at the frontier between plant and pathogen and help determine the outcome of the interaction, it is critical to understand their functions. Here, we used a direct proteomics approach to identify effector candidates from P. triticina Race 1 haustoria isolated with a specific monoclonal antibody. Haustoria were >95% pure and free of host contaminants. Using high resolution MS we have identified 1192 haustoria proteins. These were quantified using normalized spectral counts and spanned a dynamic range of three orders of magnitude, with unknown proteins and metabolic enzymes as the most highly represented. The …
Anahtar Kelimeler
Basidiomycete | Brown rust | Plant proteomics | Puccinia recondita f. sp. tritici
BM Sürdürülebilir Kalkınma Amaçları
Atıf Sayıları
Google Scholar 20
Scopus 1
The proteome of monoclonal antibody purified haustoria from Puccinia triticina Race 1

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