Sub-cellular proteomics for deep understanding
Yazarlar (1)
Prof. Dr. Aslıhan GÜNEL
Kırşehir Ahi Evran Üniversitesi, Türkiye
| Makale Türü | Özgün Makale (Diğer hakemli uluslarası dergilerde yayınlanan tam makale) | ||
| Dergi Adı | Journal of Systems Biology and Proteome Reseaerch | ||
| Dergi ISSN | 1234-5678 | ||
| Makale Dili | İngilizce | Basım Tarihi | 02-2018 |
| Cilt / Sayı / Sayfa | 2 / 1 / 8–8 | DOI | – |
| Özet |
| Protein functions have acquired more importance since the completion of the first genome studies in 2000’s. We have learned that we have more proteins than genome size while the introduction of omics technologies and their development in the course of time have shown that the protein profiles of the organisms are changeable under different stimuli. Since the first introduction of the term “proteome”, a number of studies on proteomics have been carried out especially in animal samples due to the less complex sample preparation methods. However, plant and plant-microbe interaction in proteomics studies was delayed due to their calcitrant nature. Proteomics studies involve several applications such as descriptive proteomics, differential proteomics, determination of Post Translational Modifications of proteins, secretomics and interaction proteomics. The knowledge obtained from these approaches is invaluable while highly abundant and generally cytosolic proteins can be identified with these approaches. Maximum 10-12000 proteins have been identified with gel free techniques and this number is lower with the gel-based techniques. Levels of some effector/elicitor proteins, disease markers, and emerged membrane receptors under different stress circumstances such as PAMPs/MAMPs are below the detection limit in total cell lysates. Enrichment of organelles and identification of proteins belonging to organelles and mining of yielded protein data by bioinformatics tools will provide opportunity to decrease the complexity and increase the elucidated proteins and underlying mechanisms of the stress conditions. Sub-cellular proteomics studies … |
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