| Yazarlar |
Fikriye Milletli Sezgin
Kırşehir Ahi Evran Üniversitesi, Türkiye |
|
Mustafa Avcu
Kırşehir Ahi Evran Üniversitesi, Türkiye |
Prof. Dr. Elif SEVİM
Kırşehir Ahi Evran Üniversitesi, Türkiye |
Doç. Dr. Ülken Tunga BABAOĞLU
Kırşehir Ahi Evran Üniversitesi, Türkiye |
| Özet |
| Objective: Staphylococcus aureus (S. aureus) is a significant pathogen microorganism that can lead to serious infections. In this study, we researchedthe activity of biofilm formation and fosfomycin on biofilm in community-acquired S. aureus isolates that were drawn from human noses.Methods: Microtitration plate method was used to determine biofilm formation. The effect of fosfomycin on sessile cells was studied on biofilmmatrix composed around plastic beads. The icaA, icaD, icaB, icaC, bap, eno, fnbA, fnbB, clfA, clfB, fib, ebpS, cna and mecA genes were screened byPolymerase Chain Reactions (PCR).Results: S. aureus was isolated from 87 samples (13.2%) out of a total 658 nasal samples. We found that 10 of these isolates (11.4%) were methicillinresistant S. aureus (MRSA). A total of 86 isolates had the ability to form biofilm. The biofilm inhibitor concentration (BIC) and minimum biofilmeradication concentration (MBEC) of fosfomycin were determined as 8 µg/ml and 32 µg/ml, respectively. In the molecular detection results ofbiofilm-related genes of these isolates, ica-dependent genes were determined to be quite high. However, no bap gene was observed to be positivein any of the isolates. Among the other genes, the most frequent genes to be declared positive were eno (97.6%) and fnbA (94.1%).Conclusion: This study indicates that prevalence of biofilm genes in S. aureus isolates in nasal flora is high and fosfomycin is an effective anti-biofilmagent alone. However, to increase fosfomycin’s efficiency, there is a need for more combination studies to make it more effective. |
| Anahtar Kelimeler |
| Staphylococcus aureus, biofilm, fosfomycin, biofilm-related genes, nasal colonisation |
| Makale Türü | Özgün Makale |
| Makale Alt Türü | ESCI dergilerinde yayımlanan tam makale |
| Dergi Adı | Clinical and Experimental Health Sciences |
| Dergi ISSN | 2459-1459 |
| Dergi Tarandığı Indeksler | ESCI |
| Makale Dili | İngilizce |
| Basım Tarihi | 09-2019 |
| Cilt No | 9 |
| Sayı | 3 |
| Sayfalar | 202 / 209 |
| Doi Numarası | 10.33808/clinexphealthsci.599855 |
| Makale Linki | http://dergipark.org.tr/doi/10.33808/clinexphealthsci.599855 |
| Atıf Sayıları |
