A new method for screening glutathione reductase inhibitors using square wave voltammetry
Yazarlar (5)
Mehmet Sayim Karacan Gazi Üniversitesi, Türkiye
Doç. Dr. Turgay TUNÇ Kırşehir Ahi Evran Üniversitesi, Türkiye
Hatice Oruç Gazi Üniversitesi, Türkiye
Serhat Mamaş
Gazi Üniversitesi, Türkiye
Gazi Üniversitesi, Türkiye
Nurcan Karacan
Gazi Üniversitesi, Türkiye
Gazi Üniversitesi, Türkiye
| Makale Türü | Özgün Makale (SSCI, AHCI, SCI, SCI-Exp dergilerinde yayınlanan tam makale) | ||
| Dergi Adı | Analytical Methods | ||
| Dergi ISSN | 1759-9660 Wos Dergi Scopus Dergi | ||
| Dergi Tarandığı Indeksler | SCI-Expanded | ||
| Makale Dili | İngilizce | Basım Tarihi | 01-2015 |
| Cilt / Sayı / Sayfa | 7 / 12 / 5142–5148 | DOI | 10.1039/c5ay00347d |
| Makale Linki | http://xlink.rsc.org/?DOI=C5AY00347D | ||
| Özet |
| A square wave voltammetric method was developed for the detection of glutathione reductase (GR) activity. The method is based upon the direct determination of glutathione (GSH) produced by nicotinamide adenine dinucleotide phosphate (NADPH)-dependent reduction of glutathione disulfide (GSSG). Enzyme activity was represented by an increase in steady-state reduction current of GSH and this current was monitored voltammetrically. At the optimized working condition, reduction potential of GSH was found at −0.44 V with a hanging mercury drop electrode versus an Ag/AgCl electrode. The reduction current was directly proportional to GSH concentration in the range 2.63–800 μM with a lower detection limit of 0.79 μM and lower quantification limit of 2.63 μM. Inhibitory activities of four antimony(III) compounds were determined by this method, and obtained IC50 values were compared with previous data. In … |
| Anahtar Kelimeler |
BM Sürdürülebilir Kalkınma Amaçları
| Atıf Sayıları | |
| Google Scholar | 8 |
| Scopus | 7 |