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Direct Bulblet Regeneration from Sternbergia fischeriana herb Rupr Bulb Scale Explants       
Yazarlar
Süleyman Kızıl
Dicle Üniversitesi, Türkiye
Khalıd M.Khawar Bhattı
Ankara Üniversitesi, Türkiye
Altuntaş Çiğdem
Prof. Dr. Sevil SAĞLAM YILMAZ
Ahi Evran Üniversitesi, Türkiye
Özet
Attractive golden yellow flowered Sternbergia fischeriana (Herb.) Rupr. multiplies very slow under natural conditions. The study reports multiplication of plants using 0.5, 1.0, and 1.5 cm long bulb scale explants with two, three, four, and five scales attached by a thin base plate segment. Any concentration of 2,4-D 1.0, 2.0, 3.0, 4.0, and 5.0 mg l-1 in MS medium was ineffective to induce bulblet regeneration on any explant at 15° ± 1°C. Discursive induction of one or two 0.1 cm diameter bulblets was noted at 24 ± 1°C on 0.5 cm long twoscale explants. Variable regeneration was observed on 0.5, 1.0, and 1.5 cm long two-scale explants on MS basal medium containing combination of BAP and 0.2 mg l-1 NAA. Maximum number of 5.0 ± 0.5 bulblets per 0.5 cm long two-scale bulb explant was obtained on MS medium containing 8.5 mg l-1 BAP plus 0.20 mg l-1 NAA. The rooting as affected by the size of bulblet was achieved on MS medium containing 0.75 mg l-1 NAA. The highest rooting was recorded on 0.47 cm diameter bulblets with 4.3 ± 0.9 roots per bulblet and 3.7 ± 0.4 cm long roots.
Anahtar Kelimeler
Bulbous plant | In vitro micropropagation | Rooting
Makale Türü Özgün Makale
Makale Alt Türü SSCI, AHCI, SCI, SCI-Exp dergilerinde yayımlanan tam makale
Dergi Adı Propagation of Ornamental Plants
Dergi ISSN 1311-9109
Dergi Tarandığı Indeksler SCI-Expanded
Makale Dili İngilizce
Basım Tarihi 05-2014
Sayfalar 68 / 75
BM Sürdürülebilir Kalkınma Amaçları
Atıf Sayıları
WoS 1
SCOPUS 2
Google Scholar 2

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