Effect Of Age On Somatic Embryogenesis From Immature Zygotic Embryos Of 5 Turkish Triticale Genotypes
Yazarlar (6)
Mehmet Atak Mustafa Kemal Üniversitesi, Türkiye
Muharrem Kaya Süleyman Demirel Üniversitesi, Türkiye
Khalıd M.Khawar Bhattı
Ankara Üniversitesi, Türkiye
Prof. Dr. Sevil SAĞLAM YILMAZ Ahi Evran Üniversitesi, Türkiye
Sebahattin Özcan Ankara Üniversitesi, Türkiye
Cemalettin Yaşar Çiftci
Ankara Üniversitesi, Türkiye
Makale Türü Açık Erişim Özgün Makale (SSCI, AHCI, SCI, SCI-Exp dergilerinde yayınlanan tam makale)
Dergi Adı African Journal of Biotechnology
Dergi ISSN 1684-5315
Dergi Tarandığı Indeksler SCI-Expanded
Makale Dili İngilizce Basım Tarihi 01-2008
Kabul Tarihi 12-04-2026 Yayınlanma Tarihi
Cilt / Sayı / Sayfa 7 / 11 / 1765–1768 DOI 10.5897/ajb08.056
Özet
Triticale is an important cereal crop grown throughout the world. The study reports somatic embryogenesis from immature zygotic embryos of 5 Turkish triticale genotypes. The explants were initially cultured on MS medium supplemented with 2 mg dm-3 2, 4-D, 500 mg dm-3 glutamine, 100 mg dm-3 casein hydrolysate, 2% sucrose and 7% agar for two weeks in the dark. The temperature was maintained at 24±2ºC. Thereafter, the developing embryogenic calli were transferred to MS medium without 2, 4-D to achieve embryogenesis under light intensity of 30 000 lux in 16 h light 8 h dark photoperiod at 24±2ºC for 2 weeks. The developing somatic embryos were then transferred to MS medium to mature them and obtain plantlets. The highest number of 9.63 somatic embryos per explant were recorded for genotype LAD388 from 14-16 days old explants. The experiment clearly established that 14-16 days old explants were superior compared to 17-19 days old explants in terms of number of somatic embryos per explant. The matured plantlets were acclimatised in the greenhouse on organic matter rich soil mix contained in pots.
Anahtar Kelimeler
Age | Immature zygotic embryos | Somatic embryogenesis | Triticale
BM Sürdürülebilir Kalkınma Amaçları
Atıf Sayıları
Google Scholar 13
Scopus 1
Web of Science 6
Effect Of Age On Somatic Embryogenesis From Immature Zygotic Embryos Of 5 Turkish Triticale Genotypes

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