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In Vitro Regeneration Of Turkish Dwarf Chickling (Lathyrus cicera L.) From Longitudinally Sliced Cotyledon Node Explant     
Yazarlar (1)
Prof. Dr. Sevil SAĞLAM YILMAZ Prof. Dr. Sevil SAĞLAM YILMAZ
Kırşehir Ahi Evran Üniversitesi, Türkiye
Devamını Göster
Özet
The forage legume cicera chickling (Lathyrus cicera (L.) DC) distributed in Mediterranean region is gaining importance in terms of economy and agriculture in Turkey. However, full potential of the legume has to be realized yet due to the presence of neurotoxin,-N-oxalyl-la,-diaminopropionoc acid (ODAP) causing lathyrism. This study aimed to develop efficient micropropagation system using longitudinally sliced cotyledon node explants for use in Agrobacterium mediated genetic transformation in the future. In this study, instead of the agar as a gelling agent alternative gelling agent isubgol was used. The results showed that the maximum number of shoots per explant was achieved on MS medium solidified with 10 g/l isubgol gelled medium containing 0.60 mg/l BAP–0.2 mg/l NAA. Shoots were rooted by pulse treatment with 30 mg/l IBA for 5 min followed by culture on 10 g/l isubgol gel solidified MS medium. The …
Anahtar Kelimeler
Makale Türü Diğer (Teknik, not, yorum, vaka takdimi, editöre mektup, özet, kitap krıtiği, araştırma notu, bilirkişi raporu ve benzeri)
Makale Alt Türü SCI, SSCI, AHCI, SCI-Exp dergilerinde yayınlanan teknik not, editöre mektup, tartışma, vaka takdimi ve özet türünden makale
Dergi Adı JOURNAL OF BIOTECHNOLOGY
Dergi ISSN 0168-1656 Wos Dergi Scopus Dergi
Dergi Tarandığı Indeksler SCI-Expanded
Makale Dili İngilizce
Basım Tarihi 11-2012
Cilt No 161
Sayfalar 16 / 16
Doi Numarası 10.1016/j.jbiotec.2012.07.025