In Vitro Regeneration Of Turkish Dwarf Chickling (Lathyrus cicera L.) From Longitudinally Sliced Cotyledon Node Explant
Yazarlar (1)
Prof. Dr. Sevil SAĞLAM YILMAZ
Kırşehir Ahi Evran Üniversitesi, Türkiye
| Makale Türü | Diğer (Teknik, not, yorum, vaka takdimi, editöre mektup, özet, kitap krıtiği, araştırma notu, bilirkişi raporu ve benzeri) (SCI, SSCI, AHCI, SCI-Exp dergilerinde yayınlanan teknik not, editöre mektup, tartışma, vaka takdimi ve özet türünden makale) | ||
| Dergi Adı | JOURNAL OF BIOTECHNOLOGY | ||
| Dergi ISSN | 0168-1656 Wos Dergi Scopus Dergi | ||
| Dergi Tarandığı Indeksler | SCI-Expanded | ||
| Makale Dili | İngilizce | Basım Tarihi | 11-2012 |
| Cilt / Sayı / Sayfa | 161 / 0 / 16–16 | DOI | 10.1016/j.jbiotec.2012.07.025 |
| Özet |
| The forage legume cicera chickling (Lathyrus cicera (L.) DC) distributed in Mediterranean region is gaining importance in terms of economy and agriculture in Turkey. However, full potential of the legume has to be realized yet due to the presence of neurotoxin,-N-oxalyl-la,-diaminopropionoc acid (ODAP) causing lathyrism. This study aimed to develop efficient micropropagation system using longitudinally sliced cotyledon node explants for use in Agrobacterium mediated genetic transformation in the future. In this study, instead of the agar as a gelling agent alternative gelling agent isubgol was used. The results showed that the maximum number of shoots per explant was achieved on MS medium solidified with 10 g/l isubgol gelled medium containing 0.60 mg/l BAP–0.2 mg/l NAA. Shoots were rooted by pulse treatment with 30 mg/l IBA for 5 min followed by culture on 10 g/l isubgol gel solidified MS medium. The … |
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