| Yazarlar |
Sevil SAĞLAM YILMAZ
Kırşehir Ahi Evran Üniversitesi, Türkiye |
Khalıd M.Khawar Bhattı
Ankara Üniversitesi, Türkiye |
|
Cemalettin Yaşar Çiftci
Türkiye |
| Özet |
| Background: Seed beetles are one of the most important causes of yield loss in bean production. It is essential to develop resistant varieties in the fight against these pests. Agrobacterium-based gene transformation is the most widely used breeding method worldwide to develop insect-resistant varieties. Methods and results: Embryonic axes and plumule explants were obtained from Agrobacterium tumefciens treated mature zygotic embryos of low and high raw protein-based common bean cultivars Akman 98 and Karacaşehir 90. Agrobacterium tumefaciens contained a synthetic Bacillus thuringiensis insecticidal crystal protein gene (Bt Cry1Ab) controlled by the 35S promoter and NOS terminator sequences. The transformation event was genotype and explant dependent. The plumule explants could not withstand kanamycin-based selection pressure and died. It was possible to get two transgenic plants using embryonic axis explants of low protein cultivar Akman 98. These results were validated using GUS analysis, PCR, RT-PCR, bioassay analysis, and ELISA test from the samples taken from T0 and T1 generations. Bioassay tests showed that these plants were protected from the damage of legume seed insects (Bruchus spp.). Conclusions: The results are very encouraging and may help in producing better transgenic common bean germplasm leading to safe agriculture and reducing environmental pollutions. |
| Anahtar Kelimeler |
| Bacillus thuringiensis | Beans | Bruchus sp | Insect resistance | Legume seeds |
| Makale Türü | Özgün Makale |
| Makale Alt Türü | SSCI, AHCI, SCI, SCI-Exp dergilerinde yayımlanan tam makale |
| Dergi Adı | MOLECULAR BIOLOGY REPORTS |
| Dergi ISSN | 0301-4851 |
| Dergi Tarandığı Indeksler | SCI-Expanded |
| Dergi Grubu | Q4 |
| Makale Dili | İngilizce |
| Basım Tarihi | 07-2022 |
| Cilt No | 49 |
| Sayı | 7 |
| Sayfalar | 7195 / 7203 |
| Doi Numarası | 10.1007/s11033-022-07637-1 |
| Makale Linki | http://dx.doi.org/10.1007/s11033-022-07637-1 |
| Atıf Sayıları | |
| WoS | 2 |
| SCOPUS | 3 |
| Google Scholar | 3 |
