Genetic transformation of common beans (Phaseolus vulgaris L.) through Agrobacterium tumefaciens carrying Cry1Ab gene
Yazarlar (3)
Prof. Dr. Sevil SAĞLAM YILMAZ Kırşehir Ahi Evran Üniversitesi, Türkiye
Khalıd M.Khawar Bhattı
Ankara Üniversitesi, Türkiye
Ankara Üniversitesi, Türkiye
Cemalettin Yaşar Çiftci
Ankara Üniversitesi, Türkiye
Ankara Üniversitesi, Türkiye
| Makale Türü | Özgün Makale (SSCI, AHCI, SCI, SCI-Exp dergilerinde yayınlanan tam makale) | ||
| Dergi Adı | Molecular Biology Reports (Q4) | ||
| Dergi ISSN | 0301-4851 Wos Dergi Scopus Dergi | ||
| Dergi Tarandığı Indeksler | SCI-Expanded | ||
| Makale Dili | İngilizce | Basım Tarihi | 06-2022 |
| Kabul Tarihi | 12-04-2026 | Yayınlanma Tarihi | – |
| Cilt / Sayı / Sayfa | 49 / 7 / 7195–7203 | DOI | 10.1007/s11033-022-07637-1 |
| Makale Linki | http://dx.doi.org/10.1007/s11033-022-07637-1 | ||
| Özet |
| BackgroundSeed beetles are one of the most important causes of yield loss in bean production. It is essential to develop resistant varieties in the fight against these pests. Agrobacterium-based gene transformation is the most widely used breeding method worldwide to develop insect-resistant varieties.Methods and resultsEmbryonic axes and plumule explants were obtained from Agrobacterium tumefciens treated mature zygotic embryos of low and high raw protein-based common bean cultivars Akman 98 and Karacaşehir 90. Agrobacterium tumefaciens contained a synthetic Bacillus thuringiensis insecticidal crystal protein gene (Bt Cry1Ab) controlled by the 35S promoter and NOS terminator sequences. The transformation event was genotype and explant dependent. The plumule explants could not withstand kanamycin-based selection pressure and died. It was possible to get two transgenic plants using … |
| Anahtar Kelimeler |
| Bacillus thuringiensis | Beans | Bruchus sp | Insect resistance | Legume seeds |
BM Sürdürülebilir Kalkınma Amaçları
| Atıf Sayıları | |
| Google Scholar | 4 |
| Scopus | 3 |
| Web of Science | 4 |