Effect of Doxorubicin loaded CS MNPs on cell proliferation in sensitive and Doxorubicin resistant MCF 7 cell lines
Yazarlar (7)
G. Unsoy
R. Khodadust
Prof. Dr. Serap YALÇIN AZARKAN Kırşehir Ahi Evran Üniversitesi, Türkiye
P. Mutlu
N. Taghavi Pourianazar
M. Parsian
U. Gunduz
| Bildiri Türü |
Tebliğ/Bildiri
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Bildiri Dili | İngilizce |
| Bildiri Alt Türü | Poster Olarak Sergilenen Tebliğ (Uluslararası Kongre/Sempozyum) | ||
| Bildiri Niteliği | Web of Science Kapsamındaki Kongre/Sempozyum | ||
| DOI Numarası | 10.1111/febs.12919 | ||
| Kongre Adı | FEBS EMBO 2014 | ||
| Kongre Tarihi | 30-08-2014 / 04-09-2014 | ||
| Basıldığı Ülke | Fransa | Basıldığı Şehir | Paris |
| UAK Araştırma Alanları |
Toksikoloji
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| Özet |
| Introduction The six transmembrane epithelial antigen of the prostate 1 (STEAP1) is over-expressed in several types of tumors, particularly in prostate cancer. STEAP1 can be found in the plasma membrane of epithelial cells, at cell–cell junctions, but also in the cytoplasm with less intensity. Although its physiological functions are yet to be ascertained, STEAP1 appears to take part on intra-and intercellular communication. Several studies have pointed out that STEAP1 has a great potential as an immunotherapeutic target, highlighting its role on prostate pathophysiology. It is well known that sex steroid hormones, namely dihydrotestosterone (DHT), are not only involved in maintenance of prostate physiology but also promote proliferation of prostate cancer cells. In addition, it was recently demonstrated that DHT is involved in regulation of STEAP1 gene. This way, we hypothesized that the effect of DHT on cell proliferation and apoptosis may be dependent on STEAP1 levels in cancer cells. Materials and Methods LNCaP prostate cancer cell line was cultured under an atmosphere of 5% CO2 and 37 C. To access the effects of DHT and STEAP1 on cell proliferation and apoptosis, cells were transfected with STEAP1 siRNA and either treated with 0 or 10 nM DHT for 48 h. Control group included nontransfected LNCaP cells and treated with 0 nM DHT. Cell cycle and apoptosis analysis was performed by flow cytometry, using propidium iodide and FITC annexin V staining, respectively. Cell proliferation was also investigated through MTS assay. Results and Discussion STEAP1 gene silencing with a specific siRNA appears to induce cell cycle arrest and … |
| Anahtar Kelimeler |
| Doxorubicin | drug resistance | breast cancer | cytotoxicity |
BM Sürdürülebilir Kalkınma Amaçları
| Atıf Sayıları | |
| Google Scholar | 1 |