| Yazarlar (4) |
Kayaaltı Zeliha
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Soylemez Esma
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Yalcin Serap
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Soylemezoglu Tulin
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| Özet |
| The alkaline single cell gel electrophoresis technique (SCGE), also known as the comet assay, is an evaluation method for estimating DNA damage and genotoxicity. This assay is used to measure DNA single-strand breaks, alkali-labile sites and DNA cross-linking in individual cells. Although the comet assay is a simple, sensitive, and rapid method to compare the results of previous studies, it is often difficult to interpret because of the variability among protocols. Standardisation and validation of the comet assay could provide invaluable information for the identification of hazardous substances and the risk assessment of environmental and occupational exposure. Further use of a standardised comet assay could include monitoring the effectiveness of medical treatment and the investigation of individual variation in response to DNA damage that may reflect genetic or environmental influences. In this study, the comet assay was evaluated for 4 parameters, including the tail moment (TM), tail intensity (TI), olive tail moment (OTM) and DNA tail (DNAt) length. 6 blood samples were collected from genetically unrelated healthy volunteers, and 7 different blood volume ranges (2.5 ml to 2.5 µl, diluted with PBS or RPMI) were tested for these parameters. The intraclass correlation coefficients (ICCs) were statistically significant among all 14 different test conditions (r= 0.87, p< 0.001 for TM; r= 0.86, p< 0.001 for TI; r= 0.73, p< 0.05 for OTM and r= 0.87, p< 0.001 for DNAt). In conclusion, the determination of DNA damage using the comet assay with small blood sample volumes can contribute worthwhile knowledge to future studies. |
| Anahtar Kelimeler |
| Makale Türü | Özgün Makale |
| Makale Alt Türü | Diğer hakemli uluslarası dergilerde yayınlanan tam makale |
| Dergi Adı | JOURNAL OF ADVANCES IN BIOLOGY (JAB) |
| Dergi ISSN | 2347-6893 |
| Dergi Tarandığı Indeksler | Google Scholar, WZB Berlin Social Science Center, Windows Live Academic, Academic Keys, Aol, Electronic Journals Library (University Library of Regensburg), International Society of Universal Research in Sciences, DRJI Directory of Research Journals Indexing, InfoBase Index, JournalTOCs, WorldCat, Research Bible, University of Passau, Rosenheim University, University of the Federal Armed Forces Munich, Bavarian State Library (BSB), Ludwig-Maximilians-University of Munich, Weihenstephan-Triesdorf, University of Regensburg, Wissenschaftskolleg Zu Berlin, iSEEK, Ingolstadt University, Catholic University of Eichstätt-Ingolstadt, State Library Neuburg / Donau, Eichstätt University Library, Amberg-Weiden University, Neu-Ulm University, Georg Simon Ohm University of Applied Sciences Nuremberg, University of Bayreuth, Friedrich-Alexander-University Erlangen-Nuremberg, University Library Ansbach, Otto-Friedrich-University of Bamberg, Coburg University, Julius-Maximilians-Universität Würzburg, HLB Fulda, Fulda University, University Library Giessen, Justus-Liebig-University Giessen, Library of the Technical University of Central Hesse, Technical University of Central Hesse, ROAD Directory of Open Access scholarly Resources, German National Library Of Science And Technology, Scientific Indexing Service, International Institute of Organized Research , Hochschule Hannover University of Applied sciences And Arts , Directory of Indexing, Scientific Indexing Services, German Cancer Research Center in The Helmholtz Association, Texila American University |
| Makale Dili | İngilizce |
| Basım Tarihi | 01-2014 |
| Cilt No | 3 |
| Sayı | 3 |
| Sayfalar | 234 / 241 |
