| Yazarlar |
Yalcin Serap
|
|
Unsoy Gozde
|
|
Mutlu Pelin
|
|
Khodadust Rouhollah
|
|
Gunduz Ufuk
|
| Özet |
| In this study, polyhydroxybutyrate (PHB)-coated magnetic nanoparticles (MNPs) were prepared by coprecipitation of iron salts (Fe2+ and Fe3+) by ammonium hydroxide. Characterizations of PHB-coated MNPs were performed by Fourier transform infrared spectroscopy, x-ray diffraction, dynamic light scattering, thermal gravimetric analysis, vibrating sample magnetometry, and transmission electron microscopy analyses. Doxorubicin was loaded onto PHB-MNPs, and the release efficiencies at different pHs were studied under in vitro conditions. The most efficient drug loading concentration was found about 87% at room temperature in phosphate-buffered saline (pH 7.2). The drug-loaded MNPs were stable up to 2 months in neutral pH for mimicking physiological conditions. The drug release studies were performed with acetate buffer (pH 4.5) that mimics endosomal pH. Doxorubicin (60%) released from PHB-MNPs within 65 hours. Doxorubicin-loaded PHB-MNPs were about 2.5-fold more cytotoxic as compared with free drug on resistant Michigan Cancer Foundation-7 (human breast adenocarcinoma, MCF-7) cell line (1 mu M doxorubicin) in vitro. Therefore, doxorubicin-loaded PHB-MNPs lead to overcome the drug resistance. |
| Anahtar Kelimeler |
| PHB, doxorubicin, magnetic nanoparticles, targeted drug delivery, drug resistance |
| Makale Türü | Özgün Makale |
| Makale Alt Türü | SSCI, AHCI, SCI, SCI-Exp dergilerinde yayımlanan tam makale |
| Dergi Adı | American Journal of Therapeutics |
| Dergi ISSN | 1075-2765 |
| Dergi Tarandığı Indeksler | SCI-Expanded |
| Makale Dili | İngilizce |
| Basım Tarihi | 01-2014 |
| Sayfalar | 1 / |
| Doi Numarası | 10.1097/MJT.0000000000000066 |
| Makale Linki | http://content.wkhealth.com/linkback/openurl?sid=WKPTLP:landingpage&an=00045391-900000000-99229 |
| Atıf Sayıları | |
| WoS | 19 |
| Google Scholar | 30 |
