Polyhydroxybutyrate coated magnetic nanoparticles for Doxorubicin delivery Cytotoxic effect against Doxorubicin resistant breast cancer cell line
Yazarlar (5)
Prof. Dr. Serap YALÇIN AZARKAN
Kırşehir Ahi Evran Üniversitesi, Türkiye
Middle East Technical University, Türkiye
Middle East Technical University, Türkiye
Middle East Technical University, Türkiye
Middle East Technical University, Türkiye
| Makale Türü |
Özgün Makale
(SSCI, AHCI, SCI, SCI-Exp dergilerinde yayınlanan tam makale)
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| Dergi Adı | AMERICAN JOURNAL OF THERAPEUTICS | ||
| Dergi ISSN | 1075-2765 Wos Dergi Scopus Dergi | ||
| Dergi Tarandığı Indeksler | SCI-Expanded | ||
| Makale Dili | İngilizce | Basım Tarihi | 11-2014 |
| Cilt / Sayı / Sayfa | 21 / 6 / 453–461 | DOI | 10.1097/MJT.0000000000000066 |
| Makale Linki | http://content.wkhealth.com/linkback/openurl?sid=WKPTLP:landingpage&an=00045391-900000000-99229 | ||
| Özet |
| In this study, polyhydroxybutyrate (PHB)-coated magnetic nanoparticles (MNPs) were prepared by coprecipitation of iron salts (Fe2+ and Fe3+) by ammonium hydroxide. Characterizations of PHB-coated MNPs were performed by Fourier transform infrared spectroscopy, x-ray diffraction, dynamic light scattering, thermal gravimetric analysis, vibrating sample magnetometry, and transmission electron microscopy analyses. Doxorubicin was loaded onto PHB-MNPs, and the release efficiencies at different pHs were studied under in vitro conditions. The most efficient drug loading concentration was found about 87% at room temperature in phosphate-buffered saline (pH 7.2). The drug-loaded MNPs were stable up to 2 months in neutral pH for mimicking physiological conditions. The drug release studies were performed with acetate buffer (pH 4.5) that mimics endosomal pH. Doxorubicin (60%) released from PHB-MNPs within 65 hours. Doxorubicin-loaded PHB-MNPs were about 2.5-fold more cytotoxic as compared with free drug on resistant Michigan Cancer Foundation-7 (human breast adenocarcinoma, MCF-7) cell line (1 mu M doxorubicin) in vitro. Therefore, doxorubicin-loaded PHB-MNPs lead to overcome the drug resistance. |
| Anahtar Kelimeler |
| PHB | doxorubicin | magnetic nanoparticles | targeted drug delivery | drug resistance |
BM Sürdürülebilir Kalkınma Amaçları
| Atıf Sayıları | |
| WoS | 20 |
| Google Scholar | 32 |