APOBEC3B expression in drug resistant MCF 7 breast cancer cell lines
Yazarlar (6)
Prof. Dr. Önder Öngürü Sağlık Bilimleri Üniversitesi, Türkiye
Prof. Dr. Serap YALÇIN AZARKAN Kırşehir Ahi Evran Üniversitesi, Türkiye
Cinthia Rosemblit
University of Pennsylvania
University of Pennsylvania
Paul Zhang
Prof. Dr. Selim Kılıç Sağlık Bilimleri Üniversitesi, Türkiye
Ufuk Gündüz
Orta Doğu Teknik Üniversitesi, Türkiye
Orta Doğu Teknik Üniversitesi, Türkiye
| Makale Türü |
Özgün Makale
(SSCI, AHCI, SCI, SCI-Exp dergilerinde yayınlanan tam makale)
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| Dergi Adı | BIOMEDICINE & PHARMACOTHERAPY | ||
| Dergi ISSN | 0753-3322 Wos Dergi Scopus Dergi | ||
| Dergi Tarandığı Indeksler | SCI-Expanded | ||
| Makale Dili | İngilizce | Basım Tarihi | 04-2016 |
| Cilt / Sayı / Sayfa | 79 / 0 / 87–92 | DOI | 10.1016/j.biopha.2016.02.004 |
| Makale Linki | http://linkinghub.elsevier.com/retrieve/pii/S0753332216301056 | ||
| UAK Araştırma Alanları |
Tıbbi Farmakoloji
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| Özet |
| APOBEC3B belongs to a protein family of cytidine deaminases that can insert mutations in DNA and RNA as a result of their ability to deaminate cytidine to uridine. It has been shown that APOBEC3B-catalysed deamination provides a chronic source of DNA damage in breast cancers. We investigated APOBEC3B expression in four drug resistant breast cancer cell lines (Doxorubicin, Etoposide, Paclitaxel and Docetaxel resistant MCF-7 cell lines) using a novel RNA in situ hybridization technology (RNAscope) and compared expression levels with drug sensitive MCF-7 cell line. After RNAscope staining, slides were scanned and saved as digital images using Aperio scanner and software. Quantitative scoring utilizing the number of punctate dots present within each cell boundary was performed for the parameters including positive cell percentage and signal intensity per positive cell. In Doxorubicin and Etoposide … |
| Anahtar Kelimeler |
| APOBEC3B | Drug resistant | MCF-7 | Breast cancer |
BM Sürdürülebilir Kalkınma Amaçları
| Atıf Sayıları |