Doxorubicin-loaded magnetic nanoparticles downregulate expression of antiapoptoticgenes in resistant breast cancer cells
Yazarlar (5)
Prof. Dr. Serap YALÇIN AZARKAN
Kırşehir Ahi Evran Üniversitesi, Türkiye
Pelin Mutlu
Orta Doğu Teknik Üniversitesi, Türkiye
Gözde Ünsoy
Başkent Üniversitesi, Türkiye
Rouhollah Khodadust
Sağlık Bilimleri Üniversitesi, Türkiye
Ufuk Gündüz
Türkiye
| Makale Türü | Özgün Makale (SCOPUS dergilerinde yayınlanan tam makale) | ||
| Dergi Adı | Journal ofMaterials NanoScience | ||
| Dergi ISSN | 2394-0867 | ||
| Dergi Tarandığı Indeksler | Scopus, chemical abstract | ||
| Makale Dili | İngilizce | Basım Tarihi | 06-2020 |
| Özet |
| In this research, surface of magnetic nanoparticles (IO-NPs) were coated with dextran polymer (Dex-IO-NPs) in which dextran provides both cavities for drug loading and drug stability. Dex-IO-NPs were synthesized by co-precipitation of iron salts with ammonium hydroxide in the presence of dextran solution. Dex-IO-NPs were then characterized by FTIR, TGA, TEM, SEM and VSM analyses. Doxorubicin was loaded on Dex-IO-NPs (Dox-Dex-IO-NPs) and applied to breast cancer (MCF-7) and Doxorubicin resistant (MCF-7/1000nM-Dox) breast cancer cell lines. Dex-IO-NPs were highly internalized and localized within the cells. Importantly, the half-maximal inhibitory concentrations (IC 50) of Dox-Dex-IO-NPs were 0.8 µM and 25 µM in MCF-7 and MCF-7/1000nMDox cells respectively, which were 2 and 7 times more effective in cell death with respect to free Doxorubicin. The release of anti-cancer agent from Dox-Dex-IO-NPs occurs with natural degradation of Dextran, and allows nuclear uptake of Doxorubicin, which results an increase in the ef cacy of Doxorubicin. The anti-apoptotic genes were downregulated in Dox-Dex-IO-NPs treated cells as compared to free Doxorubicin treated cells, revealing the higher cytotoxicity and apoptotic potential of Dox-Dex-IO-NPs. These results may imply that Dex-IO-NPs particles can have a potential to be an efficient tool for drug delivery in breast cancer therapy. |
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