| Yazarlar |
Nevin Çankaya
Uşak Üniversitesi, Türkiye |
|
Mehmetcan İzdal
|
Serap YALÇIN AZARKAN
Kırşehir Ahi Evran Üniversitesi, Türkiye |
| Özet |
| Background: In recent years, the discovery and development of new drugs play a critical role in cancer therapy. Objective: In this study, the effect of MPAEA and p-acetamide on cellular toxicity and on silico in HeLa cancer cells have been investigated. Methods: In this study, 2-choloro-N-(4-methoxyphenyl)acetamide (p-acetamide) and 2-(4methoxyphenylamino)-2-oxoethyl acrylate (MPAEA) have been synthesized and characterized by FTIR, H-1, and C-13-NMR. Cytotoxicity of p-acetamide and MPAEA have been investigated by XTT cell proliferation assay on the HeLa cell line. IC50 values of p-acetamide and MPAEA have been identified on the HeLa cell line. Further, a molecular docking study was carried out by Autodock Vina using BCL-2 (PDB ID: 4MAN), BCL-W (PDB ID: 2Y6W), MCl-1 (PDB ID: 5FDO) AKT (PDB ID: 4GV1) and BRAF (PDB ID: 5VAM) as a possible apoptotic target for anticancer activity. Results: According to the obtained results, MPAEA and p-acetamide were successfully synthesized and characterized. The interactions between ligands and anti-apoptotic proteins were evaluated by molecular docking, and their free energy of binding was calculated and used as a descriptor. Conclusion: In vitro and in silico, the results demonstrated that MPAEA had potent anticancer activity on the HeLa cell line. |
| Anahtar Kelimeler |
| Synthesis and characterization | antiproliferative activity | hela cell line | molecular docking | anti-apoptotic proteins | MPAEA |
| Makale Türü | Özgün Makale |
| Makale Alt Türü | SSCI, AHCI, SCI, SCI-Exp dergilerinde yayımlanan tam makale |
| Dergi Adı | CURRENT COMPUTER-AIDED DRUG DESIGN |
| Dergi ISSN | 1573-4099 |
| Dergi Tarandığı Indeksler | SCI |
| Makale Dili | İngilizce |
| Basım Tarihi | 01-2021 |
| Cilt No | 17 |
| Sayı | 6 |
| Sayfalar | 838 / 848 |
| Doi Numarası | 10.2174/1573409916666200907160434 |
| Makale Linki | https://www.eurekaselect.com/article/109760 |
| Atıf Sayıları | |
| WoS | 5 |
| Google Scholar | 7 |
