| Yazarlar |
Pelin Mutlu
Türkiye |
|
Murad Mutlu
Türkiye |
Prof. Dr. Serap YALÇIN AZARKAN
Kırşehir Ahi Evran Üniversitesi, Türkiye |
|
Kemal Keseroğlu
Türkiye |
|
Ömer Bayır
Türkiye |
|
Güleser Saylam
Türkiye |
|
Mehmet Hakan Korkmaz
Türkiye |
| Özet |
| Besides the variations in genomic DNA, mitochondrial DNA (mtDNA) mutations are also responsible for many diseases, including cancer. MtDNA among individuals from the same and different ethnic groups is highly polymorphic. In the present study, we screened mitochondrial CO-1 and ND4 gene sequences of Turkish head and neck squamous cell carcinoma (HNSCC) patient group and examined the possible relationship between CO-1 and ND4 gene mutations and the development of the disease. Sixty unrelated Turkish HNSCC patients and thirty six unrelated healthy volunteers from different geographic regions of Turkey were included in this study. Total DNA isolation from blood samples were carried out and amplification of CO-1 and ND4 gene regions of mtDNA were performed by PCR reaction. PCR products were purified and sequencing was carried out by Sanger sequencing. Two mutations in CO-1 gene were identified and among them A6272d mutation was found as statistically significant in the studied HNSCC patient group with respect to control group (p 0.05). Also differences in the alpha helix structure of the protein in patients with mutations were observed. Two mutations (A11251G and T11017TA) in the ND4 gene region were identified, however, none of these mutations were seem to be responsible for the disease development (p 0.05). However, we cannot detect a statistically significant alteration between patient and control groups for ND4 gene (p 0.05). These differences can be due to ethnic differences. |
| Anahtar Kelimeler |
| HNSCC, Mitochondrial DNA, CO-1 gene, ND4 gene, Turkish patient group |
| Makale Türü | Özgün Makale |
| Makale Alt Türü | SSCI, AHCI, SCI, SCI-Exp dergilerinde yayımlanan tam makale |
| Dergi Adı | UHOD-Uluslararasi Hematoloji-Onkoloji Dergisi |
| Dergi ISSN | 1306-133X |
| Dergi Tarandığı Indeksler | SCI-Expanded |
| Dergi Grubu | Q4 |
| Makale Dili | Türkçe |
| Basım Tarihi | 01-2022 |
| Doi Numarası | 10.4999/uhod.226394 |
| Makale Linki | http://www.uhod.org/pdf/PDF_908.pdf |
| Atıf Sayıları |
