The Determination of Molecular and Toxicological Mechanisms of Cucurbitacin E in Model Organism Drosophila melanogaster and Various Cancer Cell Lines: Molecular Modelling, Docking and Dynamic Simulation Studies
Yazarlar (3)
Aydın Şüzü Tunçbilek
Türkiye
Türkiye
Prof. Dr. Serap YALÇIN AZARKAN Kırşehir Ahi Evran Üniversitesi, Türkiye
Prof. Dr. Fahriye ERCAN Kırşehir Ahi Evran Üniversitesi, Türkiye
| Makale Türü | Özgün Makale (SSCI, AHCI, SCI, SCI-Exp dergilerinde yayınlanan tam makale) | ||
| Dergi Adı | CURRENT COMPUTER-AIDED DRUG DESIGN (Q3) | ||
| Dergi ISSN | 1573-4099 Dergi Bilgileri (2023) | ||
| Dergi Tarandığı Indeksler | SCI-Expanded | ||
| Makale Dili | Türkçe | Basım Tarihi | 01-2023 |
| Cilt / Sayı / Sayfa | 19 / 2 / 81–93 | DOI | 10.2174/1573409919666221031112223 |
| Makale Linki | http://dx.doi.org/10.2174/1573409919666221031112223 | ||
| UAK Araştırma Alanları |
Toksikoloji
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| Özet |
| Introduction Cucurbitacins are one of the most important components of Ecballium elaterium. Among the cucurbitacins, Cucurbitacin E was the first to be isolated. This study focused on screening the anticancer and insecticidal potential of Cucurbitacin E by the in-vitro, invivo, and in-silico methods. Methods In the study, toxicity analysis of Cucurbitacin E was determined on HeLa, Caco 2 cancer cell lines and D. melanogaster. While the expression levels of the BAD, BCL-2, AKT-1 and H-purine genes of cancer cell lines were determined, the CG15530, BUFFY, AKT-1 and Purine genes of D. melanogaster were determined by RT-PCR. Besides, molecular docking and dynamic properties of Cucurbitacin E with human and insectoid enzymes were presented in silico. Results The IC50 value of Cucurbitacin E in the HeLa ovarian and Caco 2 colon cancer cell lines was determined to be 42 ug/ml and 85 ug/ml … |
| Anahtar Kelimeler |
| Cucurbitacin E | Drosophila melanogaster | cancer | toxicity | molecular docking | molecular dynamic analyses |
BM Sürdürülebilir Kalkınma Amaçları
| Atıf Sayıları | |
| Web of Science | 1 |
| Google Scholar | 2 |