| Yazarlar |
Lakkhana Kanhayuwa
|
|
Ioly Kottaloızou
|
Doç. Dr. Selin ÖZKAN KOTİLOĞLU
Ahi Evran Üniversitesi, Türkiye |
|
Robert Ha Coutts
|
|
A Patrick Gunnıng
|
| Özet |
| We report the discovery and characterization of a double-stranded RNA (dsRNA) mycovirus isolated from the human pathogenic fungus Aspergillus fumigatus, Aspergillus fumigatus tetramycovirus-1 (AfuTmV-1), which reveals several unique features not found previously in positive-strand RNA viruses, including the fact that it represents the first dsRNA (to our knowledge) that is not only infectious as a purified entity but also as a naked dsRNA. The AfuTmV-1 genome consists of four capped dsRNAs, the largest of which encodes an RNA-dependent RNA polymerase (RdRP) containing a unique GDNQ motif normally characteristic of negative-strand RNA viruses. The third largest dsRNA encodes an S-adenosyl methionine-dependent methyltransferase capping enzyme and the smallest dsRNA a P-A-S-rich protein that apparently coats but does not encapsidate the viral genome as visualized by atomic force microscopy. A combination of a capping enzyme with a picorna-like RdRP in the AfuTmV-1 genome is a striking case of chimerism and the first example (to our knowledge) of such a phenomenon. AfuTmV-1 appears to be intermediate between dsRNA and positive-strand ssRNA viruses, as well as between encapsidated and capsidless RNA viruses. |
| Anahtar Kelimeler |
| mycovirus, infectious dsRNA, protoplast transfection, capping enzyme, virus evolution |
| Makale Türü | Özgün Makale |
| Makale Alt Türü | SSCI, AHCI, SCI, SCI-Exp dergilerinde yayımlanan tam makale |
| Dergi Adı | Proceedings of the National Academy of Sciences |
| Dergi ISSN | 0027-8424 |
| Dergi Tarandığı Indeksler | SCI-Expanded |
| Dergi Grubu | Q1 |
| Makale Dili | İngilizce |
| Basım Tarihi | 07-2015 |
| Cilt No | 112 |
| Sayı | 29 |
| Sayfalar | 9100 / 9105 |
| Doi Numarası | 10.1073/pnas.1419225112 |
| Makale Linki | http://www.pnas.org/lookup/doi/10.1073/pnas.1419225112 |
| Atıf Sayıları | |
| WoS | 105 |
| Google Scholar | 147 |
