Improvement of Decellularization Efficiency of Porcine Aorta Using Dimethyl Sulfoxide as a Penetration Enhancer
Yazarlar (4)
Dr. Öğr. Üyesi Selcan GÜLER Kırşehir Ahi Evran Üniversitesi, Türkiye
Halil M. Aydin Hacettepe Üniversitesi, Türkiye
Lan Xin Lü Keele University, School Of Medicine, İngiltere
Ying Yang Keele University, School Of Medicine, İngiltere
| Makale Türü | Özgün Makale (SSCI, AHCI, SCI, SCI-Exp dergilerinde yayınlanan tam makale) | ||
| Dergi Adı | Artificial Organs (Q2) | ||
| Dergi ISSN | 0160-564X Dergi Bilgileri (2018) | ||
| Dergi Tarandığı Indeksler | SSCI | ||
| Makale Dili | İngilizce | Basım Tarihi | 02-2018 |
| Cilt / Sayı / Sayfa | 42 / 2 / 219–230 | DOI | 10.1111/aor.12978 |
| Makale Linki | http://doi.wiley.com/10.1111/aor.12978 | ||
| UAK Araştırma Alanları |
Biyomalzemeler
|
||
| Özet |
| Decellularization of tissues and organs enables researchers to obtain extracellular matrix (ECM) with the natural conformation and chemical composition of specific tissues. However, drawbacks exist such as the structural alteration of ECM or loss of some important components in ECM due to overexposure to chemicals during the decellularization process. In this study, porcine aorta was decellularized by sodium dodecyl sulfate (SDS). Dimethyl sulfoxide (DMSO) was used as a penetration enhancer in the decellularization process to enhance the penetration of SDS, consequently reducing the exposure time of SDS to treated tissues. It is revealed that by addition of DMSO to the decellularization process 64.4% more DNA was removed when compared with just SDS exposure within a 3 h reaction. Cross‐validation by DAPI staining showed that, in the presence of DMSO, the penetration of SDS was improved and … |
| Anahtar Kelimeler |
| Aorta | Decellularization | Dimethyl sulfoxide | Penetration enhancer | Sodium dodecyl sulfate |
BM Sürdürülebilir Kalkınma Amaçları
| Atıf Sayıları | |
| Web of Science | 14 |
| Scopus | 19 |
| Google Scholar | 24 |