Influence of methionine and dithioerythritol on sperm motility, lipid peroxidation and antioxidant capacities during liquid storage of ram semen
 
Yazarlar (10)
Kenan Çoyan Selçuk Üniversitesi, Türkiye
Nuri Başpınar Selçuk Üniversitesi, Türkiye
Mustafa Numan Bucak Selçuk Üniversitesi, Türkiye
Pınar Coşkun Hatay Mustafa Kemal Üniversitesi, Türkiye
Mehmet Bozkurt Ataman
Selçuk Üniversitesi, Türkiye
Ali Doğan Ömür Selçuk Üniversitesi, Türkiye
Şükrü Güngör Selçuk Üniversitesi, Türkiye
Dr. Öğr. Üyesi Sadık KÜÇÜKGÜNAY Kırşehir Ahi Evran Üniversitesi, Türkiye
Birol Özkalp Selçuk Üniversitesi, Türkiye
Serpil Sarıözkan Erciyes Üniversitesi, Türkiye
Makale Türü Özgün Makale (SSCI, AHCI, SCI, SCI-Exp dergilerinde yayınlanan tam makale)
Dergi Adı Research in Veterinary Science
Dergi ISSN 0034-5288 Wos Dergi Scopus Dergi
Dergi Tarandığı Indeksler SCI-Expanded
Makale Dili İngilizce Basım Tarihi 01-2010
Kabul Tarihi 12-04-2026 Yayınlanma Tarihi
Cilt / Sayı / Sayfa 89 / 3 / 426–431 DOI 10.1016/j.rvsc.2010.03.025
Özet
The aim of this study was to investigate the effects of methionine and dithioerythritol, added to the Tris extender, on ram sperm motility and LPO (lipid peroxidation) and antioxidant capacities during liquid storage up to 72h at 5°C. Ejaculates collected from five Merino rams, were evaluated and pooled at 37°C. This study included two experiments. In experiment 1, each pooled ejaculate was divided into four equal aliquots and diluted (37°C) with the base extender, containing 0 (control), 1, 2 and 4mM methionine, at a final concentration of approximately 4×108sperms/ml (single step dilution), in a 15-ml plastic centrifuge tube. In experiment 2, dithioerythritol, at concentrations of 0 (control), 0.5, 1 and 2mM, was used as an additive in the extender, and the procedure explained above was applied for the division of aliquots and the dilution of semen. Diluted semen samples were kept in glass tubes and cooled from 37 to 5 …
Anahtar Kelimeler
Antioxidant capacity | Dithioerythritol | Lipid peroxidation | Liquid storage | Methionine | Ram semen