img
Somatic embryogenesis callus production and plantlet growth in sainfoin Onobrychis viciifolia Scop    
Yazarlar
Nafiz Çeliktaş
Mustafa Kemal Üniversitesi, Türkiye
Ersin Can
Mustafa Kemal Üniversitesi, Türkiye
Prof. Dr. Rüştü HATİPOĞLU Prof. Dr. Rüştü HATİPOĞLU
Çukurova Üniversitesi, Türkiye
Süleyman Avcı
Eskişehir Osmangazi Üniversitesi, Türkiye
Ömer Delialioğlu
Türkiye
Özet
Plant parts (internode segments, young leaves, apical-axillary meristems, petioles and immature inflorescences) isolated from the fresh, full-leaved and juicy sainfoin (Onobrychis viciifolia Scop.) were used as explants in this study. The explants were cultured on B5 medium with 5.4 mu M or 10.7 mu M NAA combined with 0, 0.9, 1.4, or 2.3 mu M of kinetin and 0, 0.9, 1.3, or 2.2 mu M of BAP to determine the effects of explant types and medium compositions on the callus induction and plant regeneration. Somatic embryogenesis and organogenesis, as well as direct shoot regeneration from the explants, were observed depending on the explant types and medium composition. The effect of the phytohormone type and concentration on the callus induction rate was under explant dependency. The highest frequency of shoot regeneration was achieved from apical-axillary meristems on B5 medium with 10.7 mu MNAA and 2.3 mu M kinetin.
Anahtar Kelimeler
sainfoin | phytohormones | explant | somatic embryogenesis
Makale Türü Özgün Makale
Makale Alt Türü SSCI, AHCI, SCI, SCI-Exp dergilerinde yayımlanan tam makale
Dergi Adı NEW ZEALAND JOURNAL OF AGRICULTURAL RESEARCH
Dergi ISSN 0028-8233
Dergi Tarandığı Indeksler SCI-Expanded
Makale Dili İngilizce
Basım Tarihi 12-2006
Cilt No 49
Sayı 4
Sayfalar 383 / 388
BM Sürdürülebilir Kalkınma Amaçları
Atıf Sayıları
WoS 14
Google Scholar 30

Paylaş