Yazarlar |
Nafiz Çeliktaş
Mustafa Kemal Üniversitesi, Türkiye |
Ersin Can
Mustafa Kemal Üniversitesi, Türkiye |
Prof. Dr. Rüştü HATİPOĞLU
Çukurova Üniversitesi, Türkiye |
Süleyman Avcı
Eskişehir Osmangazi Üniversitesi, Türkiye |
Ömer Delialioğlu
Türkiye |
Özet |
Plant parts (internode segments, young leaves, apical-axillary meristems, petioles and immature inflorescences) isolated from the fresh, full-leaved and juicy sainfoin (Onobrychis viciifolia Scop.) were used as explants in this study. The explants were cultured on B5 medium with 5.4 mu M or 10.7 mu M NAA combined with 0, 0.9, 1.4, or 2.3 mu M of kinetin and 0, 0.9, 1.3, or 2.2 mu M of BAP to determine the effects of explant types and medium compositions on the callus induction and plant regeneration. Somatic embryogenesis and organogenesis, as well as direct shoot regeneration from the explants, were observed depending on the explant types and medium composition. The effect of the phytohormone type and concentration on the callus induction rate was under explant dependency. The highest frequency of shoot regeneration was achieved from apical-axillary meristems on B5 medium with 10.7 mu MNAA and 2.3 mu M kinetin. |
Anahtar Kelimeler |
sainfoin | phytohormones | explant | somatic embryogenesis |
Makale Türü | Özgün Makale |
Makale Alt Türü | SSCI, AHCI, SCI, SCI-Exp dergilerinde yayımlanan tam makale |
Dergi Adı | NEW ZEALAND JOURNAL OF AGRICULTURAL RESEARCH |
Dergi ISSN | 0028-8233 |
Dergi Tarandığı Indeksler | SCI-Expanded |
Makale Dili | İngilizce |
Basım Tarihi | 12-2006 |
Cilt No | 49 |
Sayı | 4 |
Sayfalar | 383 / 388 |