| Yazarlar |
İbrahim Atış
Mustafa Kemal Üniversitesi, Türkiye |
|
Ersin Can
Mustafa Kemal Üniversitesi, Türkiye |
|
Nafiz Çeliktaş
Mustafa Kemal Üniversitesi, Türkiye |
Rüştü HATİPOĞLU
Çukurova Üniversitesi, Türkiye |
| Özet |
| This study was carried out to determine the effects of MS medium supplemented with 2, 4, 6 and 8 mg l(-1) of 2,4,5-T (2,4,5-trichlorophenoxyacetic acid) on young inflorescences of five ecotypes of orchardgrass (Dactylis glomerata L.). The results showed that there was a significant interaction between ecotypes and 2,4,5-T concentrations in all characteristics studied. Depending on the ecotypes, rate of callus induction (%), rate of shoot formation (%), callus weight (mg/per Petri dish) and number of regenerates per inflorescence segment varied and ranged 6.3-37.5%, 7.8-51.6%, 13.1-115.4 mg and 0.3-1.1, respectively. Rates of callus induction and shoot formation as well as callus weight were also significantly influenced by the 2,4,5-T concentrations. The explants cultured on MS medium containing 4 mg(-1) of 2,4,5-T showed highest callus induction rate of 53.8 % with callus weight of 170.4 mg/Petri dish and 1.6 shoots per explant. It was concluded that best regeneration from young inflorescence explant of orchardgrass (D. glomerata) could be obtained on MS medium containing 4 mg l(-1) of 2,4,5-T and the developed protocol will help in easy multiplication of orchard grass in future breeding studies. |
| Anahtar Kelimeler |
| Orchardgrass | MS medium | 2,4,5-T. | tissue culture | plant regeneration |
| Makale Türü | Özgün Makale |
| Makale Alt Türü | SSCI, AHCI, SCI, SCI-Exp dergilerinde yayımlanan tam makale |
| Dergi Adı | JOURNAL OF FOOD AGRICULTURE & ENVIRONMENT |
| Dergi ISSN | 1459-0255 |
| Dergi Tarandığı Indeksler | SCI-Expanded |
| Makale Dili | İngilizce |
| Basım Tarihi | 01-2013 |
| Cilt No | 11 |
| Sayı | 1 |
| Sayfalar | 564 / 567 |
