| Yazarlar |
Faheem Shahzad Baloch
Abant İzzet Baysal Üniversitesi, Türkiye |
|
Ahmad Alsalah
|
|
Luıs Saenz De Mıera
|
Rüştü HATİPOĞLU
Çukurova Üniversitesi, Türkiye |
|
Vahdettin Çiftçi
Abant İzzet Baysal Üniversitesi, Türkiye |
|
Tolga Karaköy
Cumhuriyet Üniversitesi, Türkiye |
|
Mehtap Yıldız
Yüzüncü Yıl Üniversitesi, Türkiye |
|
Hakan Özkan
Çukurova Üniversitesi, Türkiye |
| Özet |
| Retrotransposons have been highly studied in monocots; however retrotransposon diversity in dicot crops has not been well documented. Our objective was to assess the diversity harbored by field pea landraces using retrotranposon markers. In this research, molecular characterization of 104 landraces and 34 field pea breeding lines was assessed using newly developed iPBS-retrotransposon markers. The 12 iPBS-retrotransposon primers generated a total 106 scorable bands, and 81 of these were found to be polymorphic (76.4%), with an average of 6.75 polymorphic fragments per primer. Polymorphism information content (PIC) ranged from 0.33 to 0.84 with an average of 0.61. It was evident that field pea landraces from the same geographical region were often placed in different groups in the neighbor joining analysis, indicating that grouping based on genetic parameters was not closely related to the geographical origin. The population structure was determined by using STRUCTURE software, and three populations at K = 3 and five populations at K = 5 were identified among landraces. The plentiful diversity present in Turkish field pea landraces could be used as genetic resource in designing breeding program, and may also contribute to worldwide pea breeding programs. Our data also suggested a role of iPBS-retrotransposons as 'a universal marker' for molecular characterization of pea germplasm. (C) 2015 Elsevier Ltd. All rights reserved. |
| Anahtar Kelimeler |
| Diversity | Genetic resources | Landraces | Pea | Retrotransposons | Turkey |
| Makale Türü | Özgün Makale |
| Makale Alt Türü | SSCI, AHCI, SCI, SCI-Exp dergilerinde yayımlanan tam makale |
| Dergi Adı | BIOCHEMICAL SYSTEMATICS AND ECOLOGY |
| Dergi ISSN | 0305-1978 |
| Dergi Tarandığı Indeksler | SCI-Expanded |
| Makale Dili | Türkçe |
| Basım Tarihi | 08-2015 |
| Cilt No | 61 |
| Sayfalar | 244 / 252 |
| Doi Numarası | 10.1016/j.bse.2015.06.017 |
| Atıf Sayıları | |
| WoS | 60 |
| Google Scholar | 90 |
