Characterization of root-associated bacterial species of Leklek variety of common bean (Phaseolus sp.)
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İlknur Akça
Aslı Küçükrecep
Prof. Dr. Rüştü HATİPOĞLU Çukurova Üniversitesi, Türkiye
Makale Türü Açık Erişim Özgün Makale (Diğer hakemli uluslarası dergilerde yayınlanan tam makale)
Dergi Adı Romanian Biotechnological Letters
Dergi ISSN 1224-5984
Dergi Tarandığı Indeksler CABI
Makale Dili İngilizce Basım Tarihi 09-2021
Cilt / Sayı / Sayfa 26 / 5 / 3008–3013 DOI
Makale Linki https://doi.org/10.25083/rbl/26.5/3008-3013.
UAK Araştırma Alanları
Moleküler Biyoloji
Özet
The common bean is a valuable food source in the human diet. Leklek is a local variety of common bean (Phaseolus sp.) widely grown in Mersin's Gülnar district, but little is known about this variety. In the present study, bacterial species from root nodules of this common bean variety were identified by PCR-amplified 16S ribosomal RNA (rRNA) gene and 16S-23S ribosomal RNA (rRNA) Internal Transcribed Spacer (ITS) region and sequencing. The partial 16S rRNA gene and 16S-23S rRNA ITS region sequences were submitted to the NCBI database (accession numbers MT967369, MT968518, respectively). Amplified sequences were used to construct a phylogenetic tree. Phylogenetic analysis based on the identified sequences showed that the isolate belonged to the genus Microbacterium and was closely related to Microbacterium paraoxydans. The findings presented here will provide a clue for understanding this bacterium's role in nodule formation in Phaseolus sp.(variety Leklek).
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