| Yazarlar (12) |
Muhammad Aasım
Türkiye |
|
Ramazan Katırcı
Türkiye |
|
Faheem Shahzad Baloch
Sivas Bilim ve Teknoloji Üniversitesi, Türkiye |
|
Zemran Mustafa
Türkiye |
|
Allah Bakhsh
Türkiye |
|
Muhammed Azhar Nadeem
Sivas Bilim ve Teknoloji Üniversitesi, Türkiye |
|
Seyid Amjad Ali
Türkiye |
Prof. Dr. Rüştü HATİPOĞLU
Kırşehir Ahi Evran Üniversitesi, Türkiye |
|
Vahdettin Çiftçi
Bolu Abant İzzet Baysal Üniversitesi, Türkiye |
|
Ephrem Habyarımana
|
|
Tolga Karaköy
Türkiye |
|
Yong Suk Chung
|
| Özet |
| Common bean is considered a recalcitrant crop for in vitro regeneration and needs repeatable and efficient in vitro regeneration protocol for its improvement through biotechnological approaches. The establishment of efficient and reproducible in vitro regeneration followed by predicting and optimizing through machine learning (ML) models such as artificial neural network (ANN) algorithms was performed. Mature embryos of common bean were pretreated with 5, 10, and 20 mg/L Benzylaminopurine (BAP) for 20 days followed by isolation of plumular apices for in vitro regeneration and cultured on a post-treatment medium containing 0.25, 0.50, 1.0, and 1.50 mg/L BAP for 8 weeks. Plumular apices explants pretreated with 20 mg/L BAP exerted negative impact and resulted in minimum shoot regeneration frequency and shoot count, but produced longer shoots. . All output variables (shoot regeneration frequency, shoot counts, and shoot length) increased significantly with the enhancement of BAP concentration in the post-treatment medium. Interaction of pretreatment × post-treatment medium revealed the need for a specific combination for inducing high shoot regeneration frequency. Higher shoot count and shoot length were achieved from the interaction of 5 mg/L BAP × 1.00 mg/L BAP followed by 10 mg/L BAP ×1.50 mg/L BAP and 20 mg/L BAP × 1.50 mg/L BAP. The valuation of data through ML models revealed R2 and MSE values ranged from 0.32-0.58 (regeneration), 0.01-0.22 (shoot counts), and 0.18-0.48 for shoot length. On the other hand, The MSE values ranged from 0.0596-0.0965 for shoot regeneration, 0.0327-0.0412 for shoot count … |
| Anahtar Kelimeler |
| Makale Türü | Özgün Makale |
| Makale Alt Türü | SSCI, AHCI, SCI, SCI-Exp dergilerinde yayınlanan tam makale |
| Dergi Adı | Frontiers in Genetics |
| Dergi ISSN | 1664-8021 Wos Dergi Scopus Dergi |
| Dergi Tarandığı Indeksler | SCI-Expanded |
| Dergi Grubu | Q1 |
| Makale Dili | İngilizce |
| Basım Tarihi | 08-2022 |
| Cilt No | 13 |
| Sayı | 897696 |
| Sayfalar | 1 / 13 |
| Doi Numarası | 10.3389/fgene.2022.897696 |
| Makale Linki | http://dx.doi.org/10.3389/fgene.2022.897696 |
| Atıf Sayıları | |
| Google Scholar | 30 |
