| Yazarlar (3) |
Prof. Dr. Ramazan BEYAZ
Kırşehir Ahi Evran Üniversitesi, Türkiye |
Murat Aycan
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Mustafa Yıldız
Ankara Üniversitesi, Türkiye |
| Özet |
| In the study, three different hypocotyl explants, 0.5 cm long, were used according to the position where they were isolated from. Seven-day-old sterile seedlings having cotyledon leaves and no root system were inoculated with bacterial solution for 20min. GV2260 line of Agrobacterium tumefaciens having ‘pBIN 19’plasmid containing npt II gene and GUS reporter gene, was used in transformation studies. After inoculation, hypocotyl section of the seedlings was divided into 3 parts labeled with number 1 (part above the root), number 2 (part above number 1) and number 3 (part below cotyledon leaves) and these parts were left to cocultivation for 2 days. Then, hypocotyl segments were transferred to selection medium containing antibiotics. The presence of npt-II (neomycin phosphotransferase II) gene in transformants was confirmed by PCR analysis. It was observed that the highest transgenic plant number was … |
| Anahtar Kelimeler |
| Bildiri Türü | Tebliğ/Bildiri |
| Bildiri Alt Türü | Özet Metin Olarak Yayınlanan Tebliğ (Uluslararası Kongre/Sempozyum) |
| Bildiri Niteliği | Web of Science Kapsamındaki Kongre/Sempozyum |
| Bildiri Dili | İngilizce |
| Kongre Adı | European Biotechnology Congress |
| Kongre Tarihi | 25-05-2017 / 27-05-2017 |
| Basıldığı Ülke | |
| Basıldığı Şehir |
| Atıf Sayıları | |
| WoS | 1 |
| Google Scholar | 2 |
