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Effect of Magnetic Field on InVitro Seedling Growth and Shoot Regeneration from Cotyledon Node Explantsof LathyruschrysanthusBoiss     
Yazarlar
Anzel Bahadır
Doç. Dr. Ramazan BEYAZ
Kırşehir Ahi Evran Üniversitesi, Türkiye
Mustafa Yıldız
Özet
The stimulatory effects on germination of seeds and growth of plants of static magnetic field (MF) pre-treatments depending on MF intensity, exposure time periods, signal form, flux density, and source frequencies on plants are reported. Seed germination frequency is low due to dormancy in Lathyrus chrysanthus Boiss. from Fabaceae family, consisting of 187 taxa. Tissue culture protocol for this plant has already been optimized. This plant is also used as a model for developing alternative methods to overcome dormancy. This study was conducted to determine the effects of MF on in vitro seed germination, seedling growth, and shoot regeneration capacity of cotyledon node explants in Lathyrus chrysanthus Boiss. to obtain healthy seedlings in large quantities. The seeds of an ecotype (Diyarbakir) were subjected to 125mT MF strength for different exposure time periods (0-untreated, 24, 48, and 72h). Sterilized seeds were germinated on growth basal medium in Magenta vessels. Seed germination and seedling growth percentages were recorded after 7 and 14 days of culture initiation, whereas seedling and root lengths were noted 28 days after culture initiation. At the end of the culture, shoot regeneration percentage, shoot number per explant, highest shoot height per explant, and total shoot number per petri dish were recorded. According to the results, it could be concluded that MF treatment could clearly be used to improve germination by breaking dormancy not only in Lathyrus chrysanthus Boiss. but also other plant species. Bioelectromagnetics. 39:547-555, 2018.(c) 2018 Wiley Periodicals, Inc.
Anahtar Kelimeler
Lathyrus chrysanthus Boiss, magnetic field, germination, seedling growth, shoot regeneration
Makale Türü Özgün Makale
Makale Alt Türü SSCI, AHCI, SCI, SCI-Exp dergilerinde yayımlanan tam makale
Dergi Adı Bioelectromagnetics
Dergi ISSN 0197-8462
Dergi Tarandığı Indeksler SCI
Makale Dili İngilizce
Basım Tarihi 09-2018
Cilt No 39
Sayı 7
Sayfalar 547 / 555
BM Sürdürülebilir Kalkınma Amaçları
Atıf Sayıları
WoS 8
SCOPUS 11

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