In vitro Explant Growth and ShootRegeneration from Petioles of SugarBeet (Beta vulgaris L.) Lines atDifferent Ploidy Levels
Yazarlar (3)
| Makale Türü | Özgün Makale |
| Makale Alt Türü | Diğer hakemli uluslarası dergilerde yayınlanan tam makale |
| Dergi Adı | Journal of Sugar Beet Research |
| Dergi ISSN | 0899-1502 |
| Makale Dili | İngilizce |
| Basım Tarihi | 06-2013 |
| Cilt No | 50 |
| Sayı | 1 |
| Sayfalar | 22 / 36 |
| Özet |
| This study was carried out to compare'ELK 345'(diploid) and'CBM 315'(tetraploid) sugar beet lines with respect to in vitro explant growth, shoot regeneration capacity of petiole explants, in vitro rooting and plantlet establishment. Petioles were cul-tured on MS (Murashige and Skoog) medium supplemented with 1 mg l-1 6-benzylaminopurine (BAP) and 0.2 mg l-1 naphthaleneacetic acid (NAA). Regenerated shoots were successfully rooted on MS medium containing 3 mg l-1 indole-3-butyric acid (IBA). It was observed that petiole explants of the tetraploid line'CBM 315'gave rise to the highest scores with respect to explant fresh and dry weights, shoot regeneration percentage, shoot number per petiole, shoot length, total shoot number per Petri dish, successful rooting and plantlet establishment. Petiole explants of diploid line ('ELK 345') produced 12.61 shoots per explants while petioles of tetraploid line ('CBM’) produced 20.23 shoots per explant. |
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