| Yazarlar (7) |
Dr. Öğr. Üyesi Özlem AYDIN
Kırşehir Ahi Evran Üniversitesi, Türkiye |
Feza Korkusuz
Hacettepe Üniversitesi, Türkiye |
|
Petek Korkusuz
Hacettepe Üniversitesi, Türkiye |
|
Elif Bilgiç
Hacettepe Üniversitesi, Türkiye |
|
Mustafa Volkan Yaprakcı
Afyon Kocatepe Üniversitesi, Türkiye |
|
Ayşen Tezcaner
Orta Doğu Teknik Üniversitesi, Türkiye |
|
Dilek Keskin
Orta Doğu Teknik Üniversitesi, Türkiye |
| Özet |
| Background: Osteoarthritis (OA) is a non-inflammatory, degenerative joint disease, leading to pain, deformity and deterioration of function together with economic loss. Joint cartilage is avascular and systemic medicine may not efficiently reach/retained at the joint fluid. Doxycycline (D), a clinically used antibiotic with a matrix metalloproteinase activity, was combined with chondroitin sulfate (CS) to prevent the progression of OA. For this end, we designed doxycycline loaded and doxycycline-chondroitin sulfate co-loaded poly-εcaprolactone (PCL) based drug delivery systems that can be applied to treat OA by intraarticular injection [1].Aim: This study aims to provide a controlled local treatment approach by preventing the progression of OA and enhancing the regeneration of cartilage matrix with the use of two bioactive agents, D and CS. The efficacy of the system was investigated upon injection of the microspheres with hyaluronan (HA) into OA developed knee joints of rabbits.Materials and Methods: Doxycycline loaded (DMS) and doxycycline-chondroitin sulfate coloaded (D-CSMS) PCL (Mw of 14 kDa) microspheres were prepared using polyvinylalcohol (PVA-4%) as emulsifier. Preparation details were described elsewhere [1]. Experimental OA was established by intra-articular collagenase type II (Clostridium histolyticum, Sigma)(4 mg/ml; 456 U/mg solid) injection into the hind knee joints of local albino adult male rabbits at day 1 and 4 [2]. Microspheres (DMS and D-CSMS), sterilized by gamma irradiation, were applied once by dispersing in 0.5 ml of hyaluronan (HA). After the establishment of OA, the treatment injections (HA, DMS and D-CSMS … |
| Anahtar Kelimeler |
| Bildiri Türü | Tebliğ/Bildiri |
| Bildiri Alt Türü | Poster Olarak Sergilenen Tebliğ (Uluslararası Kongre/Sempozyum) |
| Bildiri Niteliği | Alanında Hakemli Uluslararası Kongre/Sempozyum |
| Bildiri Dili | İngilizce |
| Kongre Adı | EORS 2014 |
| Kongre Tarihi | 02-07-2014 / 04-07-2014 |
| Basıldığı Ülke | Fransa |
| Basıldığı Şehir | Nantes |
