| Yazarlar (3) |
Şeyma Şensoy Karaoğlu
|
Prof. Dr. Ali SEVİM
Kırşehir Ahi Evran Üniversitesi, Türkiye |
Prof. Dr. Elif SEVİM
Kırşehir Ahi Evran Üniversitesi, Türkiye |
| Özet |
| Bacillus amyloliquefaciens B10 that was isolated from soil was identified according to data of morphological, biochemical and 16S rRNA sequence analyses. The bacteriocin like peptide (BLP) produced by B. amyloliquefaciens B10 shows extendend antimicrobial activity against gram-positive bacteria including some pathogenic bacteria such as B. cereus and L. monocytogenesis and yeast including opportunistic pathogen such as Candida albicans. The BLP was sensitive to proteolytic enzyme proteinase K, lipase, tripsin, αamylase except for catalase (5 mg/ml). While the activity of BLP of B13 was stable at 90 C for 30 min, the activity was completely lost at 90 C for 2 h and 121 C 20 min. Although activity of BLP of B10 was stable pH range 3-9, optimum pH was 7.0. It was sensitive against some organic solvent and detergent such as ethanol, hexane, ethyl ether and tween-80. Partial purification of BLP of B10 performed 70% ammonium sulphate precipitation was shown that it has approximately 5 kDa molecular weight. |
| Anahtar Kelimeler |
| Makale Türü | Özgün Makale |
| Makale Alt Türü | Diğer hakemli ulusal dergilerde yayınlanan tam makale |
| Dergi Adı | Journal of the Institute of Science and Technology Of Erciyes University |
| Dergi ISSN | 1012-2354 |
| Makale Dili | Türkçe |
| Basım Tarihi | 11-2014 |
| Cilt No | 30 |
| Sayı | 5 |
| Sayfalar | 338 / 345 |
| Makale Linki | http://fbe.erciyes.edu.tr/MKA-2005/Dergi/2014-vol30-no-5/MAKALE6.pdf |
