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Production and partial characterization of bacteriocin like peptide produced by Bacillus amyloliquefaciens B10   
Yazarlar (3)
Şeyma Şensoy Karaoğlu
Prof. Dr. Ali SEVİM Prof. Dr. Ali SEVİM
Ahi Evran Üniversitesi, Türkiye
Prof. Dr. Elif SEVİM Prof. Dr. Elif SEVİM
Ahi Evran Üniversitesi, Türkiye
Devamını Göster
Özet
Bacillus amyloliquefaciens B10 that was isolated from soil was identified according to data of morphological, biochemical and 16S rRNA sequence analyses. The bacteriocin like peptide (BLP) produced by B. amyloliquefaciens B10 shows extendend antimicrobial activity against gram-positive bacteria including some pathogenic bacteria such as B. cereus and L. monocytogenesis and yeast including opportunistic pathogen such as Candida albicans. The BLP was sensitive to proteolytic enzyme proteinase K, lipase, tripsin, αamylase except for catalase (5 mg/ml). While the activity of BLP of B13 was stable at 90 C for 30 min, the activity was completely lost at 90 C for 2 h and 121 C 20 min. Although activity of BLP of B10 was stable pH range 3-9, optimum pH was 7.0. It was sensitive against some organic solvent and detergent such as ethanol, hexane, ethyl ether and tween-80. Partial purification of BLP of B10 performed 70% ammonium sulphate precipitation was shown that it has approximately 5 kDa molecular weight.
Anahtar Kelimeler
Makale Türü Özgün Makale
Makale Alt Türü Diğer hakemli ulusal dergilerde yayınlanan tam makale
Dergi Adı Journal of the Institute of Science and Technology Of Erciyes University
Dergi ISSN 1012-2354
Makale Dili Türkçe
Basım Tarihi 11-2014
Cilt No 30
Sayı 5
Sayfalar 338 / 345
Makale Linki http://fbe.erciyes.edu.tr/MKA-2005/Dergi/2014-vol30-no-5/MAKALE6.pdf
BM Sürdürülebilir Kalkınma Amaçları
Atıf Sayıları
Google Scholar 7

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