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Molecular characterization virulence and horizontal transmission of Beauveria pseudobassiana from Dendroctonus micans Kug Coleoptera Curculionidae       
Yazarlar
Seda Kocaçevik
Prof. Dr. Ali SEVİM
Ahi Evran Üniversitesi, Türkiye
Mahmut Eroğlu
Karadeniz Teknik Üniversitesi, Türkiye
Zihni Demirbağ
Karadeniz Teknik Üniversitesi, Türkiye
İsmail Demir
Karadeniz Teknik Üniversitesi, Türkiye
Özet
The great spruce bark beetle, Dendroctonus micans (Kugelann) (Coleoptera: Curculionidae), has been a potential threat for Turkey and the entire Eurasian spruce forests for many years. Control strategies which have been applied so far are still insufficient to prevent its damage. A previous study has shown that a Beauveria isolate (ARSEF 9271) proved to be an efficient microbial control agent against the great spruce bark beetle. In this study, this isolate was identified as B.pseudobassiana based on the partial sequence of EF1- and ITS sequence. A conidial suspension (1x10(8)/ml) of this fungus caused 100% mortality on both larvae and adults of D.micans within 5 and 6days, respectively. Also, it caused 100% mycosis value on both larvae and adults. Mortality values of horizontal transmission experiments between larvae and adults which were contaminated with 1x10(6)/ml spore suspension at 25%, 50%, 75% and 100% rates were determined as 100% after 15days at 20 degrees C under the laboratory conditions. We also determined the decrease of the damage in spruce wood block (15x25cm) when the contamination rate of the larvae was increased. Our results indicate that B.pseudobassiana ARSEF 9271 seems to be a very promising biocontrol agent against D.micans.
Anahtar Kelimeler
Beauveria pseudobassiana, Dendroctonus micans, horizontal transmission, microbial control, molecular characterization
Makale Türü Özgün Makale
Makale Alt Türü SSCI, AHCI, SCI, SCI-Exp dergilerinde yayımlanan tam makale
Dergi Adı Journal of Applied Entomology
Dergi ISSN 1439-0418
Dergi Tarandığı Indeksler SCI-Expanded
Makale Dili İngilizce
Basım Tarihi 11-2015
Cilt No 139
Sayı 5
Sayfalar 381 / 389
Doi Numarası 10.1111/jen.12181
Makale Linki http://onlinelibrary.wiley.com/doi/10.1111/jen.12181/abstract