img
Molecular Characterization and Virulence of Beauveria spp from the Pine Processionary Moth Thaumetopoea pityocampa Lepidoptera Thaumetopoeidae        
Yazarlar
Prof. Dr. Ali SEVİM
Ahi Evran Üniversitesi, Türkiye
İsmail Demir
Karadeniz Teknik Üniversitesi, Türkiye
Zihni Demirbağ
Karadeniz Teknik Üniversitesi, Türkiye
Özet
The pine processionary moth Thaumetopoea pityocampa (Den. & Schiff.) is one of the most harmful pests to pine species in Mediterranean countries including Turkey. Caterpillars of T. pityocampa are not only significantly harmful to forest trees but also responsible for various allergic reactions in humans and animals. In this study, in order to find a more effective and safe biological control agent against T. pityocampa, we investigated fungal pathogens of T. pityocampa in the Black Sea Region of Turkey and tested their pathogenicity on it. Five different fungi were isolated and identified based on their morphological and molecular characteristics including ITS and partial sequence of EF1-[alpha]. Based on these characteristics, four isolates were identified as Beauveria bassiana cf. Clade C (Rehner and Buckley in Mycologia 97:84-98, 2005) and one isolate was identified as Beauveria bassiana. Among these isolates, B. bassiana KTU-24, B. bassiana cf. Clade C KTU-66 and KTU-67 showed the highest virulence with 100% mortality within 10 days after application. B. bassiana isolate KTU-24 produced the highest mycosis value with %100. Consequently, B. bassiana KTU-24 seems to be good candidate for further investigation as a possible biological control agent against this pest.
Anahtar Kelimeler
Pine processionary moth, Fungal pathogen, Beauveria, Microbial control
Makale Türü Özgün Makale
Makale Alt Türü SSCI, AHCI, SCI, SCI-Exp dergilerinde yayımlanan tam makale
Dergi Adı Mycopathologia
Dergi ISSN 0301-486X
Dergi Tarandığı Indeksler SCI-Expanded
Makale Dili İngilizce
Basım Tarihi 01-2010
Sayı 170
Sayfalar 269 / 277
Doi Numarası 10.1007/s11046-010-9321-6
Makale Linki http://www.ncbi.nlm.nih.gov/pubmed/20499189