Yazarlar (4) |
Filiz Özkan Çakıcı
|
Prof. Dr. Ali SEVİM
Ahi Evran Üniversitesi, Türkiye |
Zihni Demirbağ
Karadeniz Teknik Üniversitesi, Türkiye |
İsmail Demir
Karadeniz Teknik Üniversitesi, Türkiye |
Özet |
Spodoptera littoralis (Boisd.) (Lepidoptera: Noctuidae) is one of the most destructive pests of several vegetables and fruitsworldwide. In spite of various control methods, this pest has still continued to cause signifcant damage. In this study, the culturablebacterial fora of S. littoralis was determined. New isolates from S. littoralis, as well as 12 diferent Bacillus isolates belong to 5 speciesthat were previously isolated from diferent pests, were tested on S. littoralis larvae. In total, 9 bacteria were characterized based ontheir morphological, biochemical, physiological, and molecular characteristics. Te bacterial fora of S. littoralis was determinedas Flavobacterium sp. (SL1), Klebsiella pneumonia (SL2), Enterobacter sp. (SL3), Enterobacter sp. (SL4), Klebsiella sp. (SL5), Serratiamarcescens (SL6), Pseudomonas aeruginosa (SL7), Acinetobacter baumannii (SL8), and Staphylococcus sp. (SL9). Te insecticidal activitytests were performed on the third-instar larvae of S. littoralis. SL1 and SL5 from S. littoralis caused the highest mortalities with 67% and77%, respectively. Among previously isolated Bacillus isolates, Bacillus thuringiensis subsp. kurstaki (MnD) and B. thuringiensis subsp.kurstaki (BnBt) were found to be the most efective, causing 100% mortality within 10 days afer treatment. A concentration-responsetest was conducted with these isolates and it was found that the isolate MnD was more efective than BnBt. Terefore, further bioassayexperiments were conducted with the isolate MnD and results were discussed with respect to the biocontrol potential of the bacterialisolates. |
Anahtar Kelimeler |
Bacillus thuringiensis | Bacterial flora | Biocontrol | Cotton leaf worm |
Makale Türü | Özgün Makale |
Makale Alt Türü | SSCI, AHCI, SCI, SCI-Exp dergilerinde yayımlanan tam makale |
Dergi Adı | TURKISH JOURNAL OF AGRICULTURE AND FORESTRY |
Dergi ISSN | 1300-011X |
Dergi Tarandığı Indeksler | SCI-Expanded |
Makale Dili | İngilizce |
Basım Tarihi | 01-2014 |
Cilt No | 38 |
Sayı | 1 |
Sayfalar | 99 / 110 |
Doi Numarası | 10.3906/tar-1302-65 |