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Investigating internal bacteria of Spodoptera littoralis Boisd Lepidoptera Noctuidae larvae and some Bacillus strains as biocontrol agents        
Yazarlar
Filiz Özkan Çakıcı
Prof. Dr. Ali SEVİM
Ahi Evran Üniversitesi, Türkiye
Zihni Demirbağ
Karadeniz Teknik Üniversitesi, Türkiye
İsmail Demir
Karadeniz Teknik Üniversitesi, Türkiye
Özet
Spodoptera littoralis (Boisd.) (Lepidoptera: Noctuidae) is one of the most destructive pests of several vegetables and fruitsworldwide. In spite of various control methods, this pest has still continued to cause signifcant damage. In this study, the culturablebacterial fora of S. littoralis was determined. New isolates from S. littoralis, as well as 12 diferent Bacillus isolates belong to 5 speciesthat were previously isolated from diferent pests, were tested on S. littoralis larvae. In total, 9 bacteria were characterized based ontheir morphological, biochemical, physiological, and molecular characteristics. Te bacterial fora of S. littoralis was determinedas Flavobacterium sp. (SL1), Klebsiella pneumonia (SL2), Enterobacter sp. (SL3), Enterobacter sp. (SL4), Klebsiella sp. (SL5), Serratiamarcescens (SL6), Pseudomonas aeruginosa (SL7), Acinetobacter baumannii (SL8), and Staphylococcus sp. (SL9). Te insecticidal activitytests were performed on the third-instar larvae of S. littoralis. SL1 and SL5 from S. littoralis caused the highest mortalities with 67% and77%, respectively. Among previously isolated Bacillus isolates, Bacillus thuringiensis subsp. kurstaki (MnD) and B. thuringiensis subsp.kurstaki (BnBt) were found to be the most efective, causing 100% mortality within 10 days afer treatment. A concentration-responsetest was conducted with these isolates and it was found that the isolate MnD was more efective than BnBt. Terefore, further bioassayexperiments were conducted with the isolate MnD and results were discussed with respect to the biocontrol potential of the bacterialisolates.
Anahtar Kelimeler
Cotton leaf worm, bacterial flora, biocontrol, Bacillus thuringiensis
Makale Türü Özgün Makale
Makale Alt Türü SSCI, AHCI, SCI, SCI-Exp dergilerinde yayımlanan tam makale
Dergi Adı Turkish Journal of Agriculture and Forestry
Dergi ISSN 1300-011X
Dergi Tarandığı Indeksler SCI-Expanded
Makale Dili İngilizce
Basım Tarihi 01-2014
Sayı 38
Sayfalar 99 / 110
Doi Numarası 10.3906/tar-1302-65