Measurement of TS-131, a New Monopyridinium Oxime, by High Performance Liquid Chromatography in Rat Plasma Samples
Yazarlar (14)
Gülhane Eğitim ve Araştırma Hastanesi, Türkiye
Gülhane Eğitim ve Araştırma Hastanesi, Türkiye
Gülhane Eğitim ve Araştırma Hastanesi, Türkiye
Gülhane Eğitim ve Araştırma Hastanesi, Türkiye
Gülhane Eğitim ve Araştırma Hastanesi, Türkiye
Gülhane Eğitim ve Araştırma Hastanesi, Türkiye
Gülhane Eğitim ve Araştırma Hastanesi, Türkiye
Gülhane Eğitim ve Araştırma Hastanesi, Türkiye
Dr. Öğr. Üyesi Nuriye Tuna SUBAŞI
Middle East Technical University (Metu), Türkiye
Gülhane Eğitim ve Araştırma Hastanesi, Türkiye
Gülhane Eğitim ve Araştırma Hastanesi, Türkiye
Middle East Technical University (Metu), Türkiye
Gülhane Eğitim ve Araştırma Hastanesi, Türkiye
Univerzita Obrany V Brne, Çek Cumhuriyeti
| Makale Türü | Özgün Makale (SCOPUS dergilerinde yayınlanan tam makale) | ||
| Dergi Adı | Journal of Clinical and Analytical Medicine | ||
| Dergi ISSN | 1309-0720 Wos Dergi Scopus Dergi | ||
| Dergi Tarandığı Indeksler | Citefactor, DOAJ, Elsevier, EMBASE, SCOPUS, Index Copernicus, Pleksus Medline, TÜBİTAK ULAKBİM, Türk Tıp DiziniTürkiye Atıf Dizini | ||
| Makale Dili | İngilizce | Basım Tarihi | 05-2011 |
| Cilt / Sayı / Sayfa | 2 / 2 / 19–21 | DOI | 10.4328/JCAM.238 |
| Makale Linki | http://dx.doi.org/10.4328/jcam.238 | ||
| Özet |
| Aim TS-131 is a monopyridinium aldoxime-type cholinesterase reactivator developed as a potential alternative to commercially available oximes. A sensitive, simple and reliable high performance liquid chromatography (HPLC) method with diode array detector was developed for the measurement of TS-131 concentrations in rat plasma samples. Material and Methods Male Spraque Dawley rats were treated intramuscularly with TS-131 and the samples were collected 30 min. later. Separation was carried out by HPLC using octadecyl silica stationary phase and a mobile phase consisting of 92% 0.1 M ammonium acetate and 8% methanol. Measurements were carried out at 40 0C. Quantitative absorbance was monitored at 242 nm. Results The calibration curve was linear through the range of 0.78-3200 μmol/L, which is well beyond the detected plasma level range of TS-131. Limit of quantitation was 0.39 μmol/L. Intra-day and inter-day precisions of the HPLC determinations gave standard deviations as 1.94 and 1.22%, respectively. After spiking, average spike recoveries ranged from 99.2% to 100.4% and, overall mean recovery of 99.8% was found. Conclusion A sensitive, simple and reliable HPLC method was established for the measurement of TS-131. The presented HPLC method with diyote arrray detector can also be used to determine the concentrations of several pyridinium oximes. |
| Anahtar Kelimeler |
| Cholinesterase Reactivator | HPLC | Oxime | Rat | TS-131 |
BM Sürdürülebilir Kalkınma Amaçları
| Atıf Sayıları | |
| SCOPUS | 1 |
| Google Scholar | 3 |