| Yazarlar (11) |
Zeki İlker Kunak
|
|
Emin Özgür Akgül
Türkiye |
|
Hakan Yaren
Türkiye |
|
Tuncer Çaycı
Türkiye |
|
Yasemin Gülcan Kurt
Türkiye |
|
Halil Yaman
|
Dr. Öğr. Üyesi Nuriye Tuna SUBAŞI
Ahi Evran Üniversitesi, Türkiye |
|
Enis Macit
Türkiye |
|
Ayhan S Demir
|
|
Mehmet Kemal Erbil
|
|
Jana Zdarova Karasova
|
| Özet |
| Aim TS-131 is a monopyridinium aldoxime-type cholinesterase reactivator developed as a potential alternative to commercially available oximes. A sensitive, simple and reliable high performance liquid chromatography (HPLC) method with diode array detector was developed for the measurement of TS-131 concentrations in rat plasma samples. Material and Methods Male Spraque Dawley rats were treated intramuscularly with TS-131 and the samples were collected 30 min. later. Separation was carried out by HPLC using octadecyl silica stationary phase and a mobile phase consisting of 92% 0.1 M ammonium acetate and 8% methanol. Measurements were carried out at 40 0C. Quantitative absorbance was monitored at 242 nm. Results The calibration curve was linear through the range of 0.78-3200 μmol/L, which is well beyond the detected plasma level range of TS-131. Limit of quantitation was 0.39 μmol/L. Intra-day and inter-day precisions of the HPLC determinations gave standard deviations as 1.94 and 1.22%, respectively. After spiking, average spike recoveries ranged from 99.2% to 100.4% and, overall mean recovery of 99.8% was found. Conclusion A sensitive, simple and reliable HPLC method was established for the measurement of TS-131. The presented HPLC method with diyote arrray detector can also be used to determine the concentrations of several pyridinium oximes. |
| Anahtar Kelimeler |
| Cholinesterase Reactivator | HPLC | Oxime | Rat | TS-131 |
| Makale Türü | Özgün Makale |
| Makale Alt Türü | SCOPUS dergilerinde yayımlanan tam makale |
| Dergi Adı | Journal of Clinical and Analytical Medicine |
| Dergi ISSN | 1309-0720 |
| Dergi Tarandığı Indeksler | Citefactor, DOAJ, Elsevier, EMBASE, SCOPUS, Index Copernicus, Pleksus Medline, TÜBİTAK ULAKBİM, Türk Tıp DiziniTürkiye Atıf Dizini |
| Makale Dili | İngilizce |
| Basım Tarihi | 05-2011 |
| Cilt No | 2 |
| Sayı | 2 |
| Sayfalar | 19 / 21 |
| Doi Numarası | 10.4328/jcam.238 |
| Makale Linki | http://dx.doi.org/10.4328/jcam.238 |
| Atıf Sayıları | |
| SCOPUS | 1 |
| Google Scholar | 3 |
