| Yazarlar |
Osman Birol Özgümüş
Recep Tayyip Erdoğan Üniversitesi, Türkiye |
|
Cemal Sandallı
Recep Tayyip Erdoğan Üniversitesi, Türkiye |
Prof. Dr. Ali SEVİM
Ahi Evran Üniversitesi, Türkiye |
Prof. Dr. Elif SEVİM
Ahi Evran Üniversitesi, Türkiye |
|
Nuket Sivri
|
| Özet |
| We aimed to determine the molecular mechanisms of antibiotic resistance in coliforms isolated from ten rivers in northern region of Turkey. A total of 183 isolates were tested for antimicrobial susceptibility by disk diffusion and agar dilution methods. Resistance to ampicillin, streptomycin, trimethoprim, tetracycline, and chloramphenicol was detected in 58%, 51.9%, 24%, 28.4%, and 12.5%, respectively. Twelve (6.5%) phylogenetically distant organisms were detected to harbor self-transmissible plasmids ranging 52 to > 147 kb in sizes. Resistances to ampicillin, tetracycline, trimethoprim, streptomycin, and nalidixic acid were commonly transferable traits. Transferable nalidixic acid-resistant strains harbored qnrS gene, which was the first report of plasmid-mediated quinolone resistance in bacteria of environmental origin in Turkey. Fourteen and five coliforms harbored class 1 and class 2 integrons, respectively, and some of them were located on transferable plasmids. Sequence analyses of variable regions of the class 1 and 2 integrons harbored various gene cassettes, dfrA1, dfr2d, dfrA7, dfrA16, dfrA17, aadA1, aadA5, bla (oxA-30), and sat1. A gene cassette array, dfrA16 has been demonstrated for the first time in a Citrobacter koseri isolate. Class 1 and class 2-bearing strains were clustered in different groups by BOX-PCR fingerprinting. Rivers in the northern Turkey may act as receptacle for the multi-drug resistant enterobacteria and can serve as reservoirs of the antimicrobial resistance determinants in the environment. The actual risk to public health is the transfer of resistance genes from the environmental bacteria to human pathogens. |
| Anahtar Kelimeler |
| Coliform | Integron | Resistance plasmid | River |
| Makale Türü | Özgün Makale |
| Makale Alt Türü | SSCI, AHCI, SCI, SCI-Exp dergilerinde yayımlanan tam makale |
| Dergi Adı | J Microbiol |
| Dergi ISSN | 1225-8873 |
| Dergi Tarandığı Indeksler | SCI-Expanded |
| Makale Dili | İngilizce |
| Basım Tarihi | 02-2009 |
| Cilt No | 47 |
| Sayı | 1 |
| Sayfalar | 19 / 27 |
| Doi Numarası | 10.1007/s12275-008-0206-z |
| Makale Linki | http://link.springer.com/article/10.1007%2Fs12275-008-0206-z?LI=true#page-1 |
| Atıf Sayıları | |
| WoS | 42 |
| SCOPUS | 41 |
| Google Scholar | 83 |
