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Isolation characterization and virulence of entomopathogenic fungi from Gryllotalpa gryllotalpa Orthoptera Gryllotalpidae       
Yazarlar
Emine Sönmez
Prof. Dr. Ali SEVİM
Ahi Evran Üniversitesi, Türkiye
Zihni Demirbağ
Karadeniz Teknik Üniversitesi, Türkiye
İsmail Demir
Karadeniz Teknik Üniversitesi, Türkiye
Özet
Mole crickets are significant pests of turf, vegetable and some tree seedlings worldwide, and it is costly to control them. In this study, we obtained 15 fungal isolates from Gryllotalpa gryllotalpa L. (Orthoptera: Gryllotalpidae) and compared the efficacy of these isolates against this pest with the aim of identifying their biocontrol potential. The fungal isolates were identified based on their morphological and molecular characteristics, using ITS, EF1-alpha, Bloc, RPB1, RPB2 and beta-Tubulin gene sequencing. Consequently, the isolates were identified as Beauveria bassiana (Balsamo) Vuillemin (Gg-1), Clonostachys sp. (Gg-2, Gg-3, Gg-5, Gg-6, Gg-8 and Gg-13), Bionectria sp. (Gg-4), Metarhizium anisopliae (Metschnikoff) Sorokin (Gg-7, Gg-12 and Gg-14), Clonostachys rogersoniana (Schroers) (Gg-9 and Gg-15), Myriodontium sp. (Gg-10) and Myriodontium keratinophilum (Samson and Polon.) (Gg-11). These isolates caused mortalities ranging from 0 to 87 %, with the most virulent fungus being M. anisopliae Gg-12, which caused 87 % mortality within 15 days post inoculation using a conidial concentration of 1 x 10(7) conidia ml(-1). Concentration-response test was conducted to determine the LC50 value of M. anisopliae Gg-12, and it was calculated as 1.069 x 10(6) conidia ml(-1). As a result, M. anisopliae Gg-12 can be further investigated in terms of controlling mole crickets.
Anahtar Kelimeler
Mole crickets, Entomopathogenic fungi, Microbial control, Metarhizium anisopliae
Makale Türü Özgün Makale
Makale Alt Türü SSCI, AHCI, SCI, SCI-Exp dergilerinde yayımlanan tam makale
Dergi Adı Applied Entomology and Zoology
Dergi ISSN 0003-6862
Dergi Tarandığı Indeksler SCI-Expanded
Makale Dili İngilizce
Basım Tarihi 05-2016
Cilt No 51
Sayfalar 213 / 223
Doi Numarası 10.1007/s13355-015-0390-3