Generation of Chimeric ''ABS Nanohemostat'' Complex and Comparing Its Histomorphological In Vivo Effects to the Traditional Ankaferd Hemostat in Controlled Experimental Partial Nephrectomy Model

Huri, Emre; Beyazit, Yavuz; Astarcı, Hesna Müzeyyen; Haznedaroğlu, İbrahim Celalettin; Mammadov, Rashad; Toksöz, Sıla; Tekinay, Ayşe Begüm; Güler, Mustafa O; Üstün, Hüseyin; Kekilli, Murat; DADALI, MÜMTAZ; Çelik, Tuğrul

doi:10.1155/2013/949460

Generation of Chimeric ''ABS Nanohemostat'' Complex and Comparing Its Histomorphological In Vivo Effects to the Traditional Ankaferd Hemostat in Controlled Experimental Partial Nephrectomy Model

Yazarlar
Emre Huri Hacettepe Üniversitesi, Türkiye
Yavuz Beyazit Çanakkale Onsekiz Mart Üniversitesi, Türkiye
Rashad Mammadov Türkiye
Sıla Toksöz
Ayşe Begüm Tekinay İhsan Doğramacı Bilkent Üniversitesi, Türkiye
Mustafa O Güler
Hüseyin Üstün Türkiye
Murat Kekilli Türkiye
Mümtaz DADALI Kurum Bilgileri Tıp Fakültesi Cerrahi Tıp Bilimleri Üroloji Özgeçmiş Sayfası İletişim Bilgileri: Ahi Evran Üniversitesi, Türkiye
Tuğrul Çelik
Hesna Müzeyyen Astarcı Abant İzzet Baysal Üniversitesi, Türkiye
İbrahim Celalettin Haznedaroğlu Hacettepe Üniversitesi, Türkiye

Özet

Purpose. Using the classical Ankaferd Blood Stopper (ABS) solution to create active hemostasis during partial nephrectomy (PN) may not be so effective due to insufficient contact surface between the ABS hemostatic liquid agent and the bleeding area. In order to broaden the contact surface, we generated a chimeric hemostatic agent, ABS nanohemostat, via combining a self-assembling peptide amphiphile molecule with the traditional Ankaferd hemostat. Materials and Methods. In order to generate ABS nanohemostat, a positively charged Peptide Amphiphile (PA) molecule was synthesized by using solid phase peptide synthesis. For animal experiments, 24 Wistar rats were divided into the following 4 groups: Group 1: control; Group 2: conventional PN with only 0.5 ml Ankaferd hemostat; Group 3: conventional PN with ABS + peptide gel; Group 4: conventional PN with only 0.5 ml peptide solution. Results. Mean warm ischemia times (WITs) were 232.8 +/- 56.3, 65.6 +/- 11.4, 75.5 +/- 17.2, and 58. 1 +/- 17.6 seconds in Group 1 to Group 4, respectively. Fibrosis was not different among the groups, while inflammation was detected to be significantly different in G3 and G4. Conclusions. ABS nanohemostat has comparable hemostatic efficacy to the traditional Ankaferd hemostat in the partial nephrectomy experimental model. Elucidation of the cellular and tissue effects of this chimeric compound may establish a catalytic spark and open new avenues for novel experimental and clinical studies in the battlefield of hemostasis.

Anahtar Kelimeler

Makale Türü	Özgün Makale
Makale Alt Türü	SCOPUS dergilerinde yayımlanan tam makale
Dergi Adı	JOURNAL OF ENDOUROLOGY
Dergi ISSN	0892-7790
Dergi Tarandığı Indeksler	PubMed, Scopus, Emerging Sources Citation, IndWeb of Scienceex
Makale Dili	İngilizce
Basım Tarihi	09-2012
Cilt No	26
Doi Numarası	10.1155/2013/949460
Makale Linki	http://dx.doi.org/10.1155/2013/949460

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Atıf Sayıları

Generation of Chimeric ''ABS Nanohemostat'' Complex and Comparing Its Histomorphological In Vivo Effects to the Traditional Ankaferd Hemostat in Controlled Experimental Partial Nephrectomy Model

Akademisyenler > Mümtaz DADALI > Yayın Detayı

Generation of Chimeric ''ABS Nanohemostat'' Complex and Comparing Its Histomorphological In Vivo Effects to the Traditional Ankaferd Hemostat in Controlled Experimental Partial Nephrectomy Model

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