| Yazarlar |
Hasan Yılmaz
Yüzüncü Yıl Üniversitesi, Türkiye |
|
Zeynep Taş Cengiz
Yüzüncü Yıl Üniversitesi, Türkiye |
Prof. Dr. Muttalip ÇİÇEK
Dicle Üniversitesi, Türkiye |
| Özet |
| Objective: To investigate the frequency of cryptosporidiosis by enzyme-linked immunosorbent assay (ELISA) and microscopy and its relationship with diarrhea. Methods: The study was prospectively performed in the Parasitology Laboratory and Pediatric Outpatient Clinic of the Research Hospital, Yuzuncu Yil University, Van, Turkey between 2004 acid 2006. Stool samples were obtained from a total of 2000 children with diarrhea, 870 females, and 1130 males aging 0-15 years as study group, and 100 children of the same age were ramdomly selected as a control group. The flotation method was firstly carried our for all stool samples in saturated zinc sulfate solution, then staining process by modified acid-first staining. All samples were also tested for Cryptosparidium parvum antigen by ELISA. Native-Lugol and trichrome staining were used to identify other intestinal parasites. Results: The antigen was determined in 97 (4.9%) of 2000 children by ELISA, however, the oocysts were only seen in 39 children (1.95%) by microscopy. Cryptosparidium spp. were not detected in the control group either by ELISA or by microscopy. We found a significant (p<0.001) relationship between diarrhea and Cryptosporidiosis. Other intestinal parasites were detected in 713 (35.7%) of 2000 diarrheic children. Conclusion: Cryptosparidium spp. antigen searching by ELISA in stool samples should be included for diagnosis of the disease in all hospitals. |
| Anahtar Kelimeler |
| Makale Türü | Özgün Makale |
| Makale Alt Türü | SSCI, AHCI, SCI, SCI-Exp dergilerinde yayımlanan tam makale |
| Dergi Adı | Saudi Med J |
| Dergi ISSN | 0379-5284 |
| Dergi Tarandığı Indeksler | SCI-Expanded |
| Makale Dili | İngilizce |
| Basım Tarihi | 01-2008 |
| Cilt No | 29 |
| Sayı | 4 |
| Sayfalar | 526 / 529 |
