The Value of micro ELISA Test in the Diagnosis of Fasciola hepatica Infection
Yazarlar (5)
Muhsin Kaya Dicle Üniversitesi, Türkiye
Remzi Beştaş Dicle Üniversitesi
Prof. Dr. Muttalip ÇİÇEK Dicle Üniversitesi, Türkiye
Akın Önder
Dicle Üniversitesi, Türkiye
Dicle Üniversitesi, Türkiye
Mehmet Ali Kaplan
Dicle Üniversitesi
Dicle Üniversitesi
| Makale Türü |
Özgün Makale
(Diğer hakemli ulusal dergilerde yayınlanan tam makale)
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| Dergi Adı | Turkiye Parazitolojii Dergisi Turkiye Parazitoloji Dernei Acta Parasitologica Turcica Turkish Society for Parasitology | ||
| Dergi ISSN | 1300-6320 Scopus Dergi | ||
| Dergi Tarandığı Indeksler | Pub Med | ||
| Makale Dili | Türkçe | Basım Tarihi | 08-2013 |
| Cilt / Sayı / Sayfa | 37 / 1 / 23–27 | DOI | 10.5152/tpd.2013.06 |
| Makale Linki | http://www.turkiyeparazitolderg.org/eng/makale/614/41/Full-Text | ||
| Özet |
| Objective: In sero-diagnosis of parasitic infection, it is essential to inspect cross-reactivity between the target parasite and other parasites in order to assess diagnostic performance. The aim of this study was to determine the cut-off value of antibody titer for diagnosis of F. hepatica (FH) infection by using the micro-ELISA and diagnostic performance of this test. Methods: The study population consisted of the following groups: FH group (n= 42), Echinococcus granulosus (EG) group (n= 27) and control group (n= 33). The micro-ELISA test for detection of anti-F. hepatica antibody was performed in all groups. Results: The test was positive in all patients with FH, in 3 out of 27 (11%) patients with EG and in none of the control group. Mean antibody titer was significantly higher in the FH group compared to the EG group (23.8±0.9 DU vs. 5.7±1.2 DU; p< 0.001) and compared to the control group (23.8±0.9 DU vs. 2.4±0.2 DU; p< 0.001). When we used 11, 5 DU as a cut-off value for sero-diagnosis of FH, the positive predictive value was 93.3%, negative predictive value was 100%, sensitivity was 100%, and specificity was 95%. Conclusion: Cross-reactions are an important issue in serological diagnosis of parasitic infections. The micro-ELISA test for FH antibody can not definitely discriminate fascioliasis from hydatid disease.(Turkiye Parazitol Derg 2013; 37: 23-7) |
| Anahtar Kelimeler |
BM Sürdürülebilir Kalkınma Amaçları
| Atıf Sayıları | |
| Google Scholar | 10 |
| Scopus | 4 |